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Volume 10, Number 11—November 2004
Dispatch

Nucleocapsid Protein as Early Diagnostic Marker for SARS

Xiao-Yan Che*Comments to Author , Wei Hao*, Yadi Wang*, Biao Di†, Kai Yin*, Yin-Chao Xu*, Chang-Sen Feng*, Zhuo-Yue Wan‡, Vincent C.C. Cheng§, and Patrick C.Y. Woo§
Author affiliations: *First Military Medical University, Guangzhou, People’s Republic of China; †Center for Disease Control and Prevention of Guangzhou, Guangzhou, People’s Republic of China; ‡Center for Disease Control and Prevention of Guangdong Province, Guangzhou, People’s Republic of China; §The University of Hong Kong, Hong Kong Special Administrative Region, People’s Republic of China

Main Article

Figure 1

N protein detection in 420 serum samples from 317 patients with severe acute respiratory syndrome (SARS). Data represent the optical density at 450 nm (OD450) of undiluted serum samples. To establish the normal range of the N protein–capture enzyme-linked immunosorbent assay, serum specimens from 400 healthy blood donors were analyzed. The mean OD450 for these specimens, as determined by the assay, was 0.078, with a standard deviation of 0.023. The cutoff OD450 of the assay was then calculated a

Figure 1. N protein detection in 420 serum samples from 317 patients with severe acute respiratory syndrome (SARS). Data represent the optical density at 450 nm (OD450) of undiluted serum samples. To establish the normal range of the N protein–capture enzyme-linked immunosorbent assay, serum specimens from 400 healthy blood donors were analyzed. The mean OD450 for these specimens, as determined by the assay, was 0.078, with a standard deviation of 0.023. The cutoff OD450 of the assay was then calculated as follows: cutoff = mean of OD450 from 400 normal sera + 5 x standard deviations = 0.19. Solid line represents cutoff value. The result was considered positive if a sample yielded OD450 above the cutoff.

Main Article

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