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Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition
Laboratory Study

Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

Shannon L. Emery*, Dean D. Erdman*Comments to Author , Michael D. Bowen*, Bruce R. Newton*, Jonas M. Winchell*, Richard F. Meyer*, Suxiang Tong*, Byron T. Cook*, Brian P. Holloway*, Karen A. McCaustland*, Paul A. Rota*, Bettina Bankamp*, Luis E. Lowe*, Tom G. Ksiazek*, William J. Bellini*, and Larry J. Anderson*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article

Table 2

Reproducibility of real-time RT-PCR assaysa

RNA transcript copy numberb
SARS1
7.5 x 101
7.5 x 102
7.5 x 103
7.5 x 104
7.5 x 105
7.5 x 106
CV within assay (%)c
2.53
0.96
0.49
0.69
1.66
0.7
CV between assays (%)d
2.39
1.09
0.82
0.64
2.1
0.79
SARS2
2.0 x 101
2 x 102
2 x 103
2 x 104
2 x 105
2 x 106
CV within assay (%)
1.27
0.57
0.46
0.72
0.84
0.67
CV between assays (%)
1.54
1.18
0.93
1.47
1.54
1.32
SARS3






CV within assay (%)
0.8
0.55
0.65
0.5
0.27
1.25
CV between assays (%) 0.94 0.64 1.07 1.13 1.24 1.65

aRT-PCR, reverse transcription–polymerase chain reaction; CV, coefficient of variation.
bTen-fold dilutions of the polymerase and nucleocapsid RNA transcripts; copies per reaction; dilution series thawed on 3 different days and assays performed in triplicate for each dilution.
cDetermined from three replicates within each assay.
dDetermined from three independent assays performed on different days.

Main Article

Page created: January 27, 2011
Page updated: January 27, 2011
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