Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition
Laboratory Study
Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus
Table 5
Results of real-time RT-PCR assay with specimens from patients with laboratory-confirmed SARS-CoV infectiona
Case ID | Location | Specimen ID | Specimen | Serology | Vero E6 culture | Conventional RT-PCRb | Real-time RT-PCR CTc values |
|||
---|---|---|---|---|---|---|---|---|---|---|
SARS1 | SARS2 | SARS3 | RNase P | |||||||
05078 |
Toronto |
2003756523 |
Lung, RM |
N/A |
– |
+ |
24.2 |
21.6 |
23 |
23.9 |
2003756525 |
Lung, RU |
– |
+ |
24.9 |
21.5 |
23 |
23.7 |
|||
05077 |
Thailand |
2003756502 |
Throat swab |
+ |
+ |
+ |
37.5 |
36.2 |
39.8 |
29.3 |
05000 |
Hong Kong |
2003757035 |
Lung, RU |
+ |
– |
+ |
26.7 |
22.6 |
24.1 |
24.7 |
2003757036 |
Lung, LU |
– |
+ |
27.2 |
24.9 |
26.5 |
26 |
|||
2003757037 |
Lung, RM |
– |
+ |
34.9 |
37.5 |
31.9 |
27.4 |
|||
2003757038 |
Lung, LL |
– |
+ |
29.6 |
27 |
28.6 |
24.5 |
|||
00220 |
Utah, USA |
2003757508 |
Sputum |
+ |
+ |
+ |
24.7 |
23 |
24.8 |
30.6 |
05001 |
Vietnam |
2003757190 |
Throat wash |
+ |
+ |
+ |
23.7 |
22.4 |
24.1 |
30.1 |
05008 |
Vietnam |
2003757229 |
Throat wash |
+ |
– |
+ |
35.5 |
35.5 |
36.7 |
30 |
05010 |
Vietnam |
2003757239 |
Throat wash |
+ |
– |
+ |
31.1 |
29.3 |
31.5 |
34.2 |
05013 |
Vietnam |
2003757251 |
Throat wash |
+ |
– |
+ |
29.5 |
28.4 |
30.3 |
28.8 |
05017 |
Vietnam |
2003757268 |
Throat wash |
+ |
+ |
+ |
26 |
24.7 |
26.4 |
27.9 |
05316 | Vietnam | 2003759760 | Throat swab | N/A | + | N/A | 25 | 25.3 | 28.2 | 28 |
aRT-PCR, reverse transcription–polymerase chain reaction; SARS-CoV, severe acute respiratory syndrome–associated coronavirus; CT, threshold cycle number; RM, right middle; RU, right upper; LU, left upper; LL, left lower; N/A, not applicable.
bRef. 5.
cValues shown mean of duplicate values.
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