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Volume 10, Number 3—March 2004

Laboratory Analysis of Tularemia in Wild-Trapped, Commercially Traded Prairie Dogs, Texas, 2002

Jeannine M. Petersen*Comments to Author , Martin E. Schriefer*, Leon G. Carter*, Yan Zhou*, Tara Sealy*, Darcy Bawiec*, Brook Yockey*, Sandra Urich*, Nordin S. Zeidner*, Swati Avashia†, Jacob L. Kool*, Jan Buck‡, Connie Lindley‡, Leos Celeda§, John A. Monteneiri*, Kenneth L. Gage*, and May C. Chu*
Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA; †Centers for Disease Control and Prevention, Atlanta, Georgia, USA; ‡Texas Dept of Health, Arlington, Texas, USA; §State Veterinary Administration, Prague, Czech Republic

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Table 2

Comparison of diagnostic sensitivities of culture and direct fluorescent assay (DFA) for detection of Francisella tularensis in live versus dead prairie dogs (groups A–C)

Prairie dogsa No. (%) of samples positive for:
Culture (spleen/liver) Direct fluorescence (spleen/liver) Direct fluorescence (lymph node) Serologic testing
Groups B, C; live, infected animals 
(n = 20)
20 (100)
10 (50)
17 (89.5)b
10 (50)
Group A, dead animals
(n = 47) 40 (85.1) 47 (100) Not tested Not tested

aAll 67 prairie dogs tested positive for F. tularensis by at least one diagnostic test (culture, DFA, or serologic testing).
b19 F. tularensis–positive animals were tested.

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