TY - JOUR AU - Guarner, Jeannette AU - Johnson, Bill J. AU - Paddock, Christopher D. AU - Shieh, Wun-Ju AU - Goldsmith, Cynthia S. AU - Reynolds, Mary G. AU - Damon, Inger K. AU - Regnery, Russell L. AU - Zaki, Sherif R. T1 - Monkeypox Transmission and Pathogenesis in Prairie Dogs T2 - Emerging Infectious Disease journal PY - 2004 VL - 10 IS - 3 SP - 426 SN - 1080-6059 AB - During May and June 2003, the first cluster of human monkeypox cases in the United States was reported. Most patients with this febrile vesicular rash illness presumably acquired the infection from prairie dogs. Monkeypox virus was demonstrated by using polymerase chain reaction in two prairie dogs in which pathologic studies showed necrotizing bronchopneumonia, conjunctivitis, and tongue ulceration. Immunohistochemical assays for orthopoxviruses demonstrated abundant viral antigens in surface epithelial cells of lesions in conjunctiva and tongue, with less amounts in adjacent macrophages, fibroblasts, and connective tissues. Viral antigens in the lung were abundant in bronchial epithelial cells, macrophages, and fibroblasts. Virus isolation and electron microscopy demonstrated active viral replication in lungs and tongue. These findings indicate that both respiratory and direct mucocutaneous exposures are potentially important routes of transmission of monkeypox virus between rodents and to humans. Prairie dogs offer insights into transmission, pathogenesis, and new vaccine and treatment trials because they are susceptible to severe monkeypox infection. KW - monkeypox virus KW - transmission KW - prairie dog KW - pathology KW - immunohistochemistry KW - electron microscopy KW - PCR KW - United States KW - mpox DO - 10.3201/eid1003.030878 UR - https://wwwnc.cdc.gov/eid/article/10/3/03-0878_article ER - End of Reference