Table 1

SARS Coronavirus Detection

Andreas Nitsche*

, Brunhilde Schweiger*, Heinz Ellerbrok*, Matthias Niedrig*, and Georg Pauli*

Author affiliations: *Robert Koch-Institut, Berlin, Germany

Primers and 5′-nuclease probes of the three SARS coronavirus–specific assays^a

Primer/probe	Primer/probe sequence	Oligonucleotide orientation	Nucleotide position^b	Tm (°C)^c
	NS pp1a (133 bp)^d
pp1a F	GCCgTAgTgTCAgTATCATCACC	S	4609–4631	56.6
pp1a R	AATAggACCAATCTCTgTAAgAgCC	A	4741–4717	56.7
pp1a TM	F-TCACTTCgTCATCAAAgACATC XT gAggAgC p	S	4661–4690	66.2
	NS pp1ab (88 bp)^d
NS F	TTTTgTTgTTTCAACTggATACCAT	S	14387–411	57.0
NS R	GAAACTgAgACgCgAgCTATgT	A	14474–453	57.3
NS TM	F-CATCCTgATTATgTACgACTCCTAAC XT CACgAA p	A	14445–413	64.4
	Surface spike glycoprotein (79 bp)^d
SS GP F	gAggTCTTTTATTgAggACTTgCTC	S	23879–903	57.1
SS GP R	gCATTCgCCATATTgCTTCAT	A	23957–937	57.3
SS GP TM	F-AAgCCAgCATCAgCgAgTgTCACCTTA XT p	A	23935–908	66.7

^aAbbreviations: SARS, severe acute respiratory syndrome; S, sense, A, antisense; F, 6-carboxyfluorescein attached to 5´-terminus (FAM); T, 5-carboxytetramethylrhodamine (5-TAMRA) attached to 5-ethylamino-dThymidin; NS, nonstructural; pp1ab, polyprotein 1ab gene; Tm, melting temperature; TM, TaqMan.
^bBased on AY274119 isolate TOR2.
^cThermodynamic Tm. All oligonucleotides were synthesized by TIB MOLBIOL Berlin.
^dAmplicon length.

Main Article

Page created: January 27, 2011

Page updated: January 27, 2011

Page reviewed: January 27, 2011

The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.

Volume 10, Number 7—July 2004

Dispatch

SARS Coronavirus Detection

Table 1