Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 10, Number 9—September 2004
Research

Experimental Infection of Ground Squirrels (Spermophilus tridecemlineatus) with Monkeypox Virus

Robert B. Tesh*Comments to Author , Douglas M. Watts*, Elena Sbrana*, Marina Siirin*, Vsevolod L. Popov*, and Shu-Yuan Xiao*
Author affiliations: *University of Texas Medical Branch, Galveston, Texas, USA

Main Article

Figure 1

Representative photomicrographs of histologic changes and immunohistochemical staining of tissues from ground squirrels infected with monkeypox virus. A) Liver from a ground squirrel (intranasal infection) showing mild degenerative changes, including early steatosis, and purple-colored viral cytoplasmic inclusion bodies in the hepatocytes (40x objective). B) Spleen from a ground squirrel infected intraperitoneally, showing extensive necrosis (20x objective). C) Liver showing positive antigen sta

Figure 1. Representative photomicrographs of histologic changes and immunohistochemical staining of tissues from ground squirrels infected with monkeypox virus. A) Liver from a ground squirrel (intranasal infection) showing mild degenerative changes, including early steatosis, and purple-colored viral cytoplasmic inclusion bodies in the hepatocytes (40x objective). B) Spleen from a ground squirrel infected intraperitoneally, showing extensive necrosis (20x objective). C) Liver showing positive antigen staining of the intrahepatocytic inclusion bodies; antigen is present in the cytoplasm and to a lesser extent in cell membranes (40x objective). D) Spleen from a ground squirrel infected intraperitoneally, showing positive antigen staining in the interstitial cells, endothelial cells (arrowheads), and the surface mesothelial cells (arrows) (20x objective). E) Same tissue sample as D, showing the edge of the spleen with antigen-positive mesothelial layer; the adjacent fat and fibrous tissue show necrosis but are also strongly positive for antigen (20x objective). F) Lung from the same animal showing many antigen-positive interstitial cells and pneumocytes (40x objective). A and B, hematoxylin and eosin stain; C–F, immunoperoxidase stain.

Main Article

Page created: March 30, 2011
Page updated: March 30, 2011
Page reviewed: March 30, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external