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Volume 11, Number 10—October 2005
Dispatch

Detecting Biological Warfare Agents

Linan Song*, Soohyoun Ahn*, and David R. Walt*Comments to Author 
Author affiliations: *Tufts University, Medford, Massachusetts, USA

Main Article

Table A3

Number of microorganisms in autoclaved bacterial cultures used as templates in polymerase chain reaction; cultures were also used to spike wastewater and prepare mixed bacterial samples*

Organism DNA copies/mL
Bacillus thuringiensis kurstaki 2.4 × 108
B. anthracis Ames 1.2 × 105
Vaccinia virus 7.7 × 106
Yersinia pestis CO-92 2.8 × 105
Clostridium botulinum A 3.1 × 107
Francisella tularensis Novicida 1.1 × 106

*Naval Medical Research Command (NMRC) did not provide an autoclaved bacterial culture of Brucella melitensis; therefore, no results were obtained for Brucella melitensis other than with synthetic target sequences. The numbers provided by NMRC in this table are based on real- time polymerase chain reaction quantification, where a standard curve was produced with cloned material and used to calculate the results of a gene copy/mL quantification of the autoclaved material.

Main Article

Page created: February 21, 2012
Page updated: February 21, 2012
Page reviewed: February 21, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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