Sudha Chaturvedi*†, Madhu Dyavaiah*, Robert A. Larsen‡§, and Vishnu Chaturvedi*†
Figure 3. Capillary electrophoresis fragment-length analyses (CE-FLA) for the identification of mating types and hybrids. The ABI PRISM 310 Genetic Analyzer and GeneScan analysis software were used for the fragment length analysis of the pheromone genes. Sense strands of MFα1 and MFa1 were labeled with fluorescent probes TET (green) and HEX (black), respectively, and polymerase chain reaction amplicons were analyzed with POP-4 polymer under denaturing conditions at 60°C. Green peak, MFα1; black peak, MFa1. These peaks were aligned by using an internal size standard, GeneScan-500 TAMRA (red peaks).