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Volume 11, Number 11—November 2005
Perspective

Emergence of Toscana Virus in Europe

Rémi N. Charrel*Comments to Author , Pierre Gallian*†, José-María Navarro-Marí‡, Loredana Nicoletti§, Anna Papa¶, Mária Paz Sánchez-Seco#, Antonio Tenorio#, and Xavier de Lamballerie*
Author affiliations: *Université de la Méditerranée, Marseille, France; †Etablissement Français du Sang Alpes-Méditerranée, Marseille, France; ‡Hospital Universitario Virgen de la Nieves, Granada, Spain; §Istituto Superiore di Sanita, Rome, Italy; ¶Aristotle University of Thessaloniki, Thessaloniki, Greece; #Instituto de Salud Carlos III, Madrid, Spain

Main Article

Table

Primers described in the literature for TOSV virus RT-PCR and nested PCR detection*

TOSV strain Primer Gene Assay Reference
TV1 5´-CCAGAGGCCATGATGAAGAAGAT-3´ N RT-PCR 14
TV2 5´-CCACTCCTATGAGCAGCTTCT-3´ N RT-PCR 14
TV3 5´-AACCTGATTTCAGTCTACCAGTT-3´ N Nested 14
TV4
 5´-TTGTTCTCAGAGATGGATTTATG-3´
 N
 Nested
 14
TosN123 5´-GAGTTTGCTTACCAAGGGTTTG-3´ N RT-PCR 37
TosN829 5´-AATCCTAATTCCCCTAACCCCC-3´ N RT-PCR 37
TosN234 5´-AACCTTGTCAGGGGNAACAAGCC-3´ N Nested 37
TosN794
 5´-GCCAACCTTGGCGCGATACTTC-3´
 N
 Nested
 37
NPhlebo1+ 5´-ATGGARGGITTTGTIWSICIICC-3´ L RT-PCR 37
Nphlebo1– 5´-AARTTRCTIGWIGCYTTIARIGTIGC-3´ L RT-PCR 37
Nphlebo2+ 5´-WTICCIAAICCIYMSAARATG-3´ L Nested 37
Nphlebo2– 5´-TCYTCYTTRTTYTTRARRTARCC-3´ L Nested 37
ATos2–
 5´-RTGRAGCTGGAAKGGIGWIG-3´
 L
 Nested†
 37
T1 5´-CTATCAACATGTCAGACGAG-3´ N RT-PCR 36
T2 5´-CGTGTCCTGTCAGAATCCCT-3´ N RT-PCR 36
T3 5´-CATTGTTCAGTTGGTCAA-3´ N Nested 36
T4 5´-CGTGTCCTGTCAGAATCCCT-3´ N Nested 36

*TOSV, Toscana virus; RT-PCR, reverse transcription–polymerase chain reaction.
†Primer used in combination with Nphlebo2+ for a nested reaction specific for TOSV.

Main Article

Page created: February 17, 2012
Page updated: February 17, 2012
Page reviewed: February 17, 2012
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