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Volume 11, Number 2—February 2005

Pneumocystis jirovecii in General Population

Francisco J. Medrano*Comments to Author , Marco Montes-Cano*, Manuel Conde*, Carmen de la Horra*, Nieves Respaldiza*, Antonia Gasch*, Maria J. Perez-Lozano*, Jose M. Varela*, and Enrique J. Calderon*
Author affiliations: *Virgen del Rocío University Hospital, Seville, Spain

Main Article


Epidemiologic, biologic, and microbiologic features of healthy Pneumocystis carriers*

Participant (age [y], sex)† Profession Alcohol intake >40 g/day Smoking habit Total lymphocyte count (cells/μL) CD4+ cell count (cells/μL) mtLSU-rRNA genotype (nucleotide position: identity) DHPS genotype (codon position: identity) by PCR-RFLP assay PCR result at month
6 of 
1 (28, F) Administrative No No 2,327 653 3 (85:T/248:C) 1 (55:Thr/57:Pro) +
2 (42, F) Administrative No No 2,455 1,168 1 (85:C/248:C) 1 (55:Thr/57:Pro)
3 (43, F) Administrative No No NA NA 4 (85:C/248:T) Not amplified
4 (40, M) Administrative Yes No 1,528 520 3 (85:T/248:C) Not amplified
5 (41, F) New resident No No NA NA 2 (85:A/248:C) 1 (55:Thr/57:Pro) NA
6 (27, F) New resident No No 1,354 550 2 (85:A/248:C) 1 (55:Thr/57:Pro)
7 (32, F) New resident No No 2,300 604 1 (85:C/248:C) 1 (55:Thr/57:Pro)
8 (40, M) University staff Yes Yes 1,730 924 2 (85:A/248:C) 1 (55:Thr/57:Pro) +
9 (26, M) University staff Yes Yes 3,602 924 2 (85:A/248:C) 1 (55:Thr/57:Pro)
10 (42, M) University staff No No 1,518 432 1 (85:C/248:C) Not amplified

*mtLSU-rRNA, mitochondrial large subunit ribosomal RNA; DHPS, dihydropteroate synthase; PCR, polymerase chain reaction; RFLP, restriction fragment length polymorphism; F, female; M, male; +, positive result; –, negative result; NA, not available.
†Chest radiograph results were normal for all participants, except participant 5, for whom results were not available, and participant 9, who showed an apical cystic bullae.

Main Article

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