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Volume 11, Number 7—July 2005
Dispatch

Cervids as Babesiae Hosts, Slovenia

Darja Duh*Comments to Author , Miroslav Petrovec*, Andrej Bidovec†, and Tatjana Avsic-Zupanc*
Author affiliations: *Institute of Microbiology and Immunology, Ljubljana, Slovenia; †Institute of Wildlife Pathology, Ljubljana, Slovenia

Main Article

Table

Nucleotide sequences and optimum annealing temperatures of the primers designed and used for nested polymerase chain reaction (PCR) and those for amplifying and sequencing the complete babesial 18S rRNA gene

Primer Nucleotide sequence (5´→3´) Annealing temperature (°C)
BABSr* CTC CAA TCC CTA GTC GGC A 60
BOD* GTT ATT GAC TCT TGT CTT TAA 53
BDV* AAT ATT GAC TGA TGT CGA GAT 53
CRYPTO F AAC CTG GTT GAT CCT GCC AGT AGT CAT 60 (6)
PIRO A* AAT ACC CAA TCC TGA CAC AGG G (7)
PIRO B* TTA AAT ACG AAT GCC CCC AAC (7)
PIRO C GTT GGG GGC ATT CGT ATT TAA 60
1055 F GGT GGT GCA TGG CCG 60
1055 R AAC GGC CAT GCA CCA C 60
1200F CAG GTC TGT GAT GCC 60
1200 R GGG CAT CAC AGA CCT G 60
CRYPTO R GAA TGA TCC TTC CGC AGG TTC ACC TAC 60 (6)

*Primers used for nested PCR.

Main Article

Page created: April 23, 2012
Page updated: April 23, 2012
Page reviewed: April 23, 2012
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