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Volume 11, Number 7—July 2005

Pandemic Vibrio parahaemolyticus O3:K6 Spread, France

Marie-Laure Quilici*Comments to Author , Annick Robert-Pillot*, Jessica Picart*, and Jean-Michel Fournier*
Author affiliations: *Institut Pasteur, Paris, France

Main Article


Characteristics of the Vibrio parahaemolyticus O3:K6 strains studied*

Strain no. CNRVC (source no.) Origin Date of isolation Source of transmission Detection of gene or phage sequences by PCR
R72H tdh trh toxRS orf8 Ribotype profile PFGE profile AP-PCR profile
970136 France (Atlantic coast) Oct 1997 Local oysters + + - + + R4† P-1a AP-a
980402 France (southwest) Sep 1998 Shellfish + + - + + R4 P-1c AP-a
990346 France (Mediterranean coast) Aug 1999 + + - + + R4 P-1c AP-a
030478 France (Atlantic coast) Aug 2003 Local shellfish + + - + + R4 UT‡ AP-a
030479 France (Atlantic coast) Aug 2003 + + - + + R4 UT AP-a
020468 (AN7410) Bangladesh 1998 + + - + + R4 P-1a AP-a
020469 (AO1851) Bangladesh 1999 + + - + + R4 P-1b AP-a
030085 (AQ4037) Maldives 1985 + - + - - Rb-2§ P-2 AP-b

*CNRVC, Centre National de Référence des Vibrions et du Cholera; PCR, polymerase chain reaction; PFGE, pulsed-field gel electrophoresis; AP-PCR, arbitrarily primed PCR.
†R4 ribotype pattern as described previously (5).
‡UT, untypeable: DNA was degraded before PFGE, presumably by DNases.
§According to our pattern designation.

Main Article

  1. Matsumoto  C, Okuda  J, Ishibashi  M, Iwanaga  M, Garg  P, Rammamurthy  T, Pandemic spread of an O3:K6 clone of Vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed PCR and toxRS sequence analyses. J Clin Microbiol. 2000;38:57885.PubMedGoogle Scholar
  2. Nishibuchi  M, Kaper  JB. Thermostable direct hemolysin gene of Vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect Immun. 1995;63:20939.PubMedGoogle Scholar
  3. Myers  ML, Panicker  G, Bej  AK. PCR detection of a newly emerged pandemic Vibrio parahaemolyticus O3:K6 pathogen in pure cultures and seeded waters from the Gulf of Mexico. Appl Environ Microbiol. 2003;69:2194200. DOIPubMedGoogle Scholar
  4. Robert-Pillot  A, Guénolé  A, Fournier  JM. Usefulness of R72H PCR assay for differentiation between Vibrio parahaemolyticus and Vibrio alginolyticus species: validation by DNA-DNA hybridization. FEMS Microbiol Lett. 2002;215:16.PubMedGoogle Scholar
  5. Bag  PK, Nandi  S, Bhadra  RK, Ramamurthy  T, Bhattacharya  SK, Nishibuchi  M, Clonal diversity among recently emerged strains of Vibrio parahaemolyticus O3:K6 associated with pandemic spread. J Clin Microbiol. 1999;37:23547.PubMedGoogle Scholar
  6. Daniels  NA, Ray  B, Easton  A, Marano  N, Kahn  E, McShan  AL, Emergence of a new Vibrio parahaemolyticus serotype in raw oysters—A prevention quandary. JAMA. 2000;284:15415. DOIPubMedGoogle Scholar
  7. McCarthy  SA, Khambaty  FM. International dissemination of epidemic Vibrio cholerae by cargo ship ballast and other nonpotable waters. Appl Environ Microbiol. 1994;60:2597601.PubMedGoogle Scholar
  8. Mead  PS, Slutsker  L, Dietz  V, McGaig  LF, Bresee  JS, Shapiro  C, Food-related illness and death in the United States. Emerg Infect Dis. 1999;5:60725. DOIPubMedGoogle Scholar
  9. Robert-Pillot  A, Guénolé  A, Lesne  J, Delesmont  R, Fournier  JM, Quilici  ML. Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France. Int J Food Microbiol. 2004;91:31925. DOIPubMedGoogle Scholar
  10. Centers for Disease Control and Prevention. Outbreak of Vibrio parahaemolyticus infections associated with eating raw oysters—Pacific Northwest, 1997. MMWR Morb Mortal Wkly Rep. 1998;47:45762.PubMedGoogle Scholar

Main Article

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