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Volume 11, Number 7—July 2005
Dispatch

SARS Coronavirus Detection Methods

Susanna K.P. Lau*, Xiao-Yan Che†, Kwok-Yung Yuen*, Beatrice H.L. Wong*, Vincent C.C. Cheng*, Gibson K.S. Woo*, Ivan F.N. Hung‡, Rosana W.S. Poon*, Kwok-Hung Chan*, J.S. Malik Peiris*, and Kwok-Yung Yuen*Comments to Author 
Author affiliations: *University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China; †First Military Medical University, Guangzhou, People's Republic of China; ‡Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China

Main Article

Table 1

Detection of SARS-CoV in clinical specimens by qRT-PCR and ELISA in relation to time from onset of symptoms*

Days from onset of symptoms No. specimens No. positive specimens (%)
qRT-PCR Polyclonal antibody–based ELISA Monoclonal antibody–based ELISA
Fecal specimens
1–5 4 3 (75) 0 1 (25)
6–10 4 3 (75) 2 (50) 0
11–15 13 9 (69) 7 (54) 5 (38)
16–20 14 13 (93) 9 (64) 7 (50)
21–25 4 3 (75) 2 (50) 1 (25)
26–30 1 1 (100) 0 0
Urine specimens
1–5 1 0 0 0
6–10 13 1 (8) 0 2 (15)
11–15 19 3 (16) 0 0
16–20 67 24 (36) 5 (7) 7 (10)
21–25 10 1 (10) 0 1 (10)
26–30 11 2 (18) 0 0
31–40 5 0 1 (20) 0
41–50 5 2 (40) 0 1 (20)
51–60 2 0 0 0

*SARS-CoV, severe acute respiratory syndrome coronavirus; qRT-PCR, quantitative reverse transcription–polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.

Main Article

Page created: April 23, 2012
Page updated: April 23, 2012
Page reviewed: April 23, 2012
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