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Volume 11, Number 7—July 2005
Research

Norovirus Recombination in ORF1/ORF2 Overlap

Rowena Bull*, Grant S. Hansman†, Leighton E. Clancy*‡, Mark M. Tanaka*, William D. Rawlinson*‡, and Peter A. White*Comments to Author 
Author affiliations: *University of New South Wales, Sydney, New South Wales, Australia; †University of Tokyo, Tokyo, Japan; ‡Prince of Wales Hospital, Randwick, New South Wales, Australia

Main Article

Figure 3

A simple mechanism for recombination in norovirus. 1) RNA transcription by the RNA-dependent RNA polymerase (RdRp) (gray circle) generates a negative-stranded intermediate (dashed line). 2) Binding of the RdRp to the almost identical RNA promoter sequences (filled boxes) generates positive-stranded (straight line) genomes and subgenomic RNA. 3) These templates direct RNA synthesis from the 3´ end that leads to the generation of both a full-length negative genome and a negative subgenomic RNA spe

Figure 3. . A simple mechanism for recombination in norovirus. 1) RNA transcription by the RNA-dependent RNA polymerase (RdRp) (gray circle) generates a negative-stranded intermediate (dashed line). 2) Binding of the RdRp to the almost identical RNA promoter sequences (filled boxes) generates positive-stranded (straight line) genomes and subgenomic RNA. 3) These templates direct RNA synthesis from the 3´ end that leads to the generation of both a full-length negative genome and a negative subgenomic RNA species. 4) Recombination occurs when the enzyme initiates positive-strand synthesis at the 3´ end of the full-length negative strand, stalls at the subgenomic promoter, and then template switches to an available negative subgenomic RNA species generated by a co-infecting virus. The net result is a recombinant virus that has acquired new open reading frame (ORF)2 and ORF3 sequences.

Main Article

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