Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 11, Number 8—August 2005
Letter

SARS Vaccine Protective in Mice

Konrad Stadler*1, Anjeanette Roberts†1, Stephan Becker‡1, Leatrice Vogel†, Markus Eickmann‡, Larissa Kolesnikova‡, Hans-Dieter Klenk‡, Brian Murphy†, Rino Rappuoli*, Sergio Abrignani*Comments to Author , and Kanta Subbarao†
Author affiliations: *Chiron Vaccines, Siena, Italy; †National Institutes of Health, Bethesda, Maryland, USA; ‡Institut für Virologie, Marburg, Germany

Main Article

Table

Immunogenicity and efficacy of β-propiolactone (BPL)–inactivated severe acute respiratory syndrome coronavirus (SARS-CoV) vaccine in mice against subsequent challenge with live SARS-CoV

Immunogen* Neutralization titer†
Virus replication upon challenge‡
Lungs
Nasal turbinates
2 wk post 1st dose 2 wk post 2nd dose 2 wk post 3rd dose No. infected/ no. tested Mean (± SE) virus titer§ No. infected/ no. tested Mean (± SE) virus titer§
PBS <1:8 <1:8 <1:8 4/4 6.3 ± 0.3 3/4 2.8 ± 0.35
MF59 <1:8 <1:8 <1:8 4/4 6.1 ± 0.13 3/4 3.0 ± 0.58
Influenza A (5 μg) <1:8 <1:8 <1:8 4/4 6.3 ± 0.07 3/4 2.9 ± 0.36
Influenza A (5 μg) + MF59 <1:8 <1:8 <1:8 4/4 6.0 ± 0.19 4/4 3.0 ± 0.11
BPL-SARS-CoV (5 μg) <1:8 <1:8 1:64 1/4 2.0 ± 0.0¶# 0/4 ≤1.8 ± 0**††
BPL-SARS-CoV (5 μg) + MF59 <1:8 1:91 1:645 0/4 ≤1.5 ± 0¶** 0/4 ≤1.8 ± 0**††

*The indicated immunogens or control preparations were administered to mice by subcutaneous injection on 3 occasions 2 weeks apart; PBS, phosphate-buffered saline.
†Neutralization titers were determined as described (8).
‡Mice were challenged with 104 50% tissue culture infectious doses (TCID50) SARS-CoV intranasally.
§Virus titers are expressed as log10 TCID50/g of tissue.
¶p<0.00001 in a 2-tailed Student t test, compared to titers seen in mice that were immunized with PBS.
#Indicates the titer of a single animal. The remaining 3 mice had no detectable levels of virus.
**Virus not detected; the lower limit of detection of infectious virus was 1.5 log10 TCID50/g in a 10% wt/vol suspension of lung homogenate and 1.8 log10 TCID50/g in a 5% wt/vol suspension of nasal turbinates.
††p = 0.025 in a 2-tailed Student t test, compared to titers seen in mice that were immunized with PBS.

Main Article

1These authors contributed equally to this work.

Page created: April 23, 2012
Page updated: April 23, 2012
Page reviewed: April 23, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external