Volume 11, Number 8—August 2005
Dispatch
Influenza A H5N1 Detection
Table 1
Primers and dual-labeled fluorescent probes of the H5 real-time reverse transcription–polymerase chain reaction assay
Primer/probe | Target | Primer/probe sequence (5´ to 3´) | Nucleotide position* |
---|---|---|---|
H5-1F | TGCCGGAATGGTCTTACATAGTG | 266–288 | |
H5-1R | HA† gene | TCTTCATAGTCATTGAAATCCCCTG | 347–323 |
H5-1P | f†-AGAAGGCCAATCCAGTCAATGACCTCTGTTA-xp† | 290–320 | |
H5-2F | GTGGCGAGCTCCCTAGCA | 1615–1632 | |
H5-2R | HA gene | TCTGCATTGTAACGACCCATTG | 1695–1674 |
H5-2P | f-TGGCAATCATGGTAGCTGGTCTATCCTTATGG-xp | 1634–1665 |
*Based on some of the Vietnam H5N1 strains (e.g., A/Viet Nam/1194/2004).
†HA, hemagglutinin; f, 6-carboxyfluorescein; xp, carboxytetramethylrhodamine.