Volume 11, Number 8—August 2005
Dispatch
Influenza A H5N1 Detection
Enders K.O. Ng*, Peter K.C. Cheng*, Antia Y.Y. Ng*, T.L. Hoang†, and Wilina W.L. Lim*
Table 1
Primers and dual-labeled fluorescent probes of the H5 real-time reverse transcription–polymerase chain reaction assay
| Primer/probe | Target | Primer/probe sequence (5´ to 3´) | Nucleotide position* |
|---|---|---|---|
| H5-1F | TGCCGGAATGGTCTTACATAGTG | 266–288 | |
| H5-1R | HA† gene | TCTTCATAGTCATTGAAATCCCCTG | 347–323 |
| H5-1P | f†-AGAAGGCCAATCCAGTCAATGACCTCTGTTA-xp† | 290–320 | |
| H5-2F | GTGGCGAGCTCCCTAGCA | 1615–1632 | |
| H5-2R | HA gene | TCTGCATTGTAACGACCCATTG | 1695–1674 |
| H5-2P | f-TGGCAATCATGGTAGCTGGTCTATCCTTATGG-xp | 1634–1665 |
*Based on some of the Vietnam H5N1 strains (e.g., A/Viet Nam/1194/2004).
†HA, hemagglutinin; f, 6-carboxyfluorescein; xp, carboxytetramethylrhodamine.
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