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Volume 11, Number 9—September 2005
Research

Molecular Epidemiology of SARS-associated Coronavirus, Beijing

Wei Liu*, Fang Tang*, Arnaud Fontanet†, Lin Zhan*, Tian-Bao Wang‡, Pan-He Zhang*, Yi-He Luan‡, Chao-Yang Cao‡, Qiu-Min Zhao*, Xiao-Ming Wu*, Zhong-Tao Xin§, Shu-Qing Zuo*, Laurence Baril†, Astrid Vabret¶, Yi-Ming Shao#, Hong Yang*, and Wu-Chun Cao*Comments to Author 
Author affiliations: *Beijing Institute of Microbiology and Epidemiology, Beijing, People's Republic of China; †Institut Pasteur, Paris, France; ‡Beijing Armed Force Hospital, Beijing, People's Republic of China; §Beijing Institute of Basic Medical Sciences, Beijing, People's Republic of China; ¶Caen University, Caen, France; #Chinese Center for Disease Control and Prevention, Beijing, People's Republic of China

Main Article

Table 1

Primers used for nested polymerase chain reaction and sequencing

Position Amplification region* Primer sets (starting from 5´)
17564 17440–18281 Forward ACGTCTATATTGGCGATCCT
TGTGCAGACTTATGAAAACAATA
Reverse GTTTTGCATTAACTCTGGTG
GTTAGTACCCACAGCATCTCTAGT
21721 21585–22304 Forward GATGATGTTCAAGCTCCTAATTAC
CTTAACAGAGCATTTGAGTTCAG
Reverse CAACATACTTCATCTATGAGGGG
TGTACCATTTTCATCATACTTGAG
22222 22177–22874 Forward AGATGTAGTTCGTGATCTACCTTC
TTAATGGCCAATAACAATTAAGA
Reverse CAAATTTTAGAGCCATTCTTACAG
GGAGAAAGGCACATTAGATATGTC
23823 23455–24263 Forward CGACACTTCTTATGAGTGCG
ATGCAGTTGATGTTGTTGTAAG
Reverse GCATTTGTGCTAGTTACCATACAG
TGATGTTGTTGTAAGTGATTCTTG
27827 27449–28270 Forward CCATCAGGAACATACGAGG
GACCACTATTGGTGTTGATTG
Reverse TAGCACACACTTTGCTTTTG
CAGTATTATTGGGTAAACCTTGG

*The nucleotide position was given with TOR2 as the reference strain (accession no. NC004718).

Main Article

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