Volume 11, Number 9—September 2005
Dispatch
West Nile Virus Detection in Kidney, Cloacal, and Nasopharyngeal Specimens
Ojimadu A. Ohajuruka*
, Richard L. Berry*, Sheila Grimes†, and Susanne Farkas*
, Richard L. Berry*, Sheila Grimes†, and Susanne Farkas*
Table
Percentage of kidney, cloacal, and nasopharyngeal swab samples with high, medium, and low positive or negative RT-PCR results (N = 100)*
| Value | High positive | Medium positive | Low positive | Total positive | Total negative |
|---|---|---|---|---|---|
| Threshold cycle (CT) range | 15.0–21.9 | 22.0–28.9 | 29.0–35.0 | CT<35.0 | CT>35.0 |
| Kidney tissues, % | 57 | 27 | 16 | 100 | 0 |
| Cloacal swabs, % | 0 | 23 | 41 | 64 | 36 |
| Nasopharyngeal swabs, % | 0 | 23 | 43 | 66 | 34 |
*RT-PCR, reverse transcription–polymerase chain reaction..
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