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Volume 12, Number 11—November 2006

Avian Influenza H5N1 Screening of Intensive Care Unit Patients with Community-acquired Pneumonia

Anucha Apisarnthanarak*Comments to Author , Pilaipan Puthavathana†, Rungrueng Kitphati‡, Pranee Thavatsupha‡, Malinee Chittaganpitch‡, Prasert Auewarakul†, and Linda M. Mundy§
Author affiliations: *Thammasart University Hospital, Pratumthani, Thailand; †Siriraj Hospital, Bangkok, Thailand; ‡Thai National Institute of Health, Nonthaburi, Thailand; §Saint Louis University School of Public Health, Saint Louis, Missouri, USA

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From February 1, 2005, to January 31, 2006, we screened 115 adults for avian influenza (H5N1) and influenza A if admitted to an intensive care unit with pneumonia. Using reverse transcription-PCR, viral culture, and serologic testing for anti-H5 antibody, we identified 8 (7%) patients with influenza A (H3N2); none had H5N1. Estimated costs for H5N1 screening were $7,375.

The ongoing avian influenza (H5N1) pandemic poses risks to both human and animal health (15). The potential exists for cross-species transmission of avian influenza to humans and subsequent reassortment of avian and human influenza viruses in coinfected persons (6). Although atypical presentations of avian influenza (H5N1) have been reported (7,8), in most H5N1 case-patients pneumonia was the primary condition (3,4). To assess the prevalence of avian influenza (H5N1) and influenza A pneumonia, we screened adults admitted to a medical intensive care unit (ICU) with community-acquired pneumonia (CAP) for H5N1 and calculated the cost estimates for H5N1 screening in a tertiary care center of an H5N1-endemic area in Thailand.

The Study

Thammasat University Hospital is a 450-bed tertiary care center with an 8-bed intensive care unit (ICU) equipped with central air-conditioning and 2 isolation rooms. The hospital serves a 150-km radius referral base in central Thailand and has 980 healthcare workers (HCWs). Annual influenza vaccination was not routinely offered to HCWs. During the study period, 2 confirmed cases of H5N1 occurred within 150 km of our hospital.

All adults admitted to the ICU with CAP between February 1, 2005, and January 31, 2006, were eligible for enrollment. Tracheal aspirates were collected for H5N1 testing, with reverse transcription (RT)-PCR, and viral culture. In patients <60 years with >14 days survival posthospitalization, paired acute-phase and convalescent-phase serum specimens were collected for identifying anti-H5 antibody. Acute-phase serum specimens for determining anti-H5 antibody were obtained within 1 week of symptoms, while convalescent-phase serum specimens were obtained >14 days after the acute-phase specimens were collected. Data collection included demographic characteristics, clinical data, and the costs associated with H5N1 screening. The diagnosis of CAP was defined according to the criteria recommended by the American Thoracic Society (9). Patients who were hospitalized for >2 days and in whom pneumonia developed were excluded from this study. The current Thai national surveillance definition for probable avian influenza (H5N1) included the following: 1) presence of fever (>38°C), and 2) influenza-like illness, and 3) exposure to sick poultry or residence in the disease-endemic areas with excess poultry death rates, and 4) radiographic evidence of severe CAP without an identified etiologic agent (10).

Viral cultures for H5N1 and influenza A, as part of screening, were incubated in Madin-Darby canine kidney (MDCK) cell monolayers at the Thai National Institute of Health. Tracheal aspirate specimens were tested by an RT-PCR assay specific for the hemagglutinin gene of H5 (11). If a specimen yielded a positive H5 band, the specimens were confirmed by different RT-PCR primers and by real-time RT-PCR (12). All serum samples were tested for H5-specific antibody by a microneutralization (micro-NT) test. The reactive samples underwent confirmatory immunofluorescence testing by using H5-transfected 293 T cells as the test antigen (13). Influenza A/Thailand/1(KAN-1)/2004 (H5N1) was used as the test virus. Acute-phase and convalescent-phase serum samples were serially diluted from 1:20 to 1:80. On the basis of previously established criteria, a positive test was defined as a neutralizing antibody titer >80 with a confirmatory immunofluorescence assay (14). Adults >60 years of age were excluded from the serologic tests because the H5N1 micro-NT was previously reported to be less specific in this population (14).

Laboratory diagnostic costs (RT-PCR for H5N1, viral culture, and paired acute- and convalescent-phase serum samples for anti-H5 antibody) for each patient were obtained from line-item reports of the hospital's fiscal system. All costs in Thai baht currency were converted to US dollars at an exchange rate of 40 bahts per 1 US dollar. The cost for isolation of the index case, if influenza A or avian influenza (H5N1) was detected, were calculated from prior cost estimates (15).

One hundred fifteen of 450 patients (25%) met the definition of CAP and consented to study participation. The patient characteristics are summarized in Table 1. None of the 115 patients had tracheal aspirates positive for H5N1; also not positive were any serologic test results from the 42 patients (37%) who were <60 years old and survived >14 days after hospitalization. We were unable to calculate the prevalence of anti-H5 antibody in this sample, given that only 37% of participants underwent complete diagnostic antibody testing.

Eighteen patients (16%) met the Thai national surveillance definition of probable H5N1, yet tracheal aspirates and serologic test results were negative for H5N1. The median time from initial symptoms to hospitalization was 4 days (range 2–8 days), and all 18 were appropriately placed on contact and droplet isolation; the mean duration of isolation was 9 days (range 4–13 days).

Although 48 (42%) of the 115 participants had no identified etiologic agent associated with CAP, Streptococcus pneumoniae (n = 39; 34%), influenza A (H3N2) (n = 8; 7%), Staphylococcus aureus (n = 7; 6%), and Haemophilus influenzae (n = 6; 5%) were the most common microorganisms detected. In addition, 19 patients (n = 19; 16%) had gram-negative microorganisms detected. All patients with H3N2 pneumonia were promptly transferred to an isolation room; 5 (62.5%) had dual infections of H3N2 and S. aureus (n = 3), Klebsiella pneumoniae (n = 1) and Pseudomonas species (n = 1), while CAP due to H3N2 developed in 3 (37.5%). Of 18 patients who met the definition of probable H5N1, 8 (44.5%) had S. pneumoniae infection, 4 (22.5%) had S. aureus infection, 2 (11%) had H3N2 infection, 2 (11%) had Burkholderia pseudomallei infection, and 2 (11%) had no other agent detected. No CAP patients had anti-H5 antibody seroconversion, although 1 participant had evidence of positive anti-H5 antibody with low titer (10) during the recovery phase. This patient lived in an avian influenza (H5N1)–endemic area without a documented excess poultry death rate, and reported no exposure to sick poultry or persons with suspected avian influenza (H5N1) infection. His tracheal culture yielded S. pneumoniae. All patients with H3N2 pneumonia sought treatment between late March and November, the influenza A season in Thailand, and were in contact and droplet isolation for a mean of 7 days (range 1–12 days). The all-cause mortality rate was 10% (Table 1). The cost estimates were $7,375 for H5N1 screening, $23,328 for subsequent infection control measures, $300 for annual influenza vaccination of ICU HCWs, and $9,800 for annual influenza vaccination of the entire hospital staff (Table 2). The perceived benefits of vaccination of all ICU HCWs included reduced risk for influenza among vaccinated HCWs and reduced risk for influenza transmission to at-risk ICU patients.


Our study findings are relevant to the prevention and control of spread of both H5N1 and H3N2. The relatively high prevalence of H3N2 (7%) among our CAP patients suggests that HCWs in ICUs in disease-endemic regions are at high-risk of acquiring influenza A. An annual influenza vaccination occupational health program, similar to those in developed countries, along with targeted case identification of patients at high risk for influenza pneumonia, may help minimize the clinical and economic consequences of influenza A transmission. Although the importance of a single patient's positive low-titer anti-H5 antibody in this study was uncertain, this finding may represent a false-positive test, given that the patient had no notable exposure to sick poultry or to persons with suspected H5N1 infection. Additionally, the fact that all 18 probable case-patients had negative results for H5N1 suggests that the current Thai surveillance definition may need further refinement. Given the potential for reassortment of H5N1 and influenza A in a coinfected person residing in a disease-endemic setting, additional H5N1 screening, along with cost-effectiveness studies, are warranted before this screening strategy is adapted to H5N1-endemic areas.

Dr Apisarnthanarak is an infectious disease specialist and hospital epidemiologist at Thammasart University Hospital, Thailand, and a member of the Thai Infection Control Work Group. His major research focus is in preventing and controlling nosocomial infections, with a secondary focus on investigating outbreaks and emerging infectious diseases.



This work was supported by a grant from National Center and Genetic Engineering and Biotechnology, National Science and Technology Development Agency (BT-01-MM-13-4806), and Thai Research Fund to A.A.



  1. World Health Organization. Cumulative number of confirmed human cases of avian influenza A (H5N1) reported to WHO, 13 February 2006 [cited 2006 Apr 1]. Available from _2006_02_13_/en/index.html
  2. Abbott  A, Pearson  H. Fear of human pandemic grows as bird flu sweeps through Asia. Nature. 2004;427:4723. DOIPubMedGoogle Scholar
  3. Chotpitayasunondh  T, Ungchusak  K, Hanshaoworakul  W, Chunsuthiwat  S, Sawanpanyalert  P, Kitphati  R, Human disease from influenza A (H5N1), Thailand, 2004. Emerg Infect Dis. 2005;11:2019.PubMedGoogle Scholar
  4. Tran  TH, Nguyen  TL, Nguyen  TD, Luong  TH, Pham  PM, Nguyen  VC, Avian influenza A (H5N1) in 10 patients in Vietnam. N Engl J Med. 2004;350:117988. DOIPubMedGoogle Scholar
  5. Apisarnthanarak  A, Erb  S, Stephenson  I, Katz  JM, Chittaganpitch  M, Sangkitporn  S, Seroprevalence of anti-H5 antibody among Thai health care workers after exposure to Avian influenza (H5N1) in a tertiary care center. Clin Infect Dis. 2005;40:e168. DOIPubMedGoogle Scholar
  6. Ungchusak  K, Auewarakul  P, Dowell  SF, Kitphati  R, Auwanit  W, Puthavathana  P, Probable person-to-person transmission of avian influenza A (H5N1). N Engl J Med. 2005;352:33340. DOIPubMedGoogle Scholar
  7. Apisarnthanarak  A, Kitphati  R, Thongphubeth  K, Patoomanant  P, Anthanont  P, Auwanit  W, Atypical avian influenza (H5N1). Emerg Infect Dis. 2004;10:13214.PubMedGoogle Scholar
  8. de Jong  MD, Bach  VC, Phan  TO, Vo  MH, Tran  TT, Smith  GJ, Fatal avian influenza A (H5N1) in a child presenting with diarrhea followed by coma. N Engl J Med. 2005;352:68691. DOIPubMedGoogle Scholar
  9. Niederman  MS, Bass  JB Jr, Campbell  GD, Fein  AM, Grossman  RF, Mandell  LA, Guidelines for the initial management of adults with community-acquired pneumonia: diagnosis, assessment of severity, and initial antimicrobial therapy. Am Rev Respir Dis. 1993;148:141826.PubMedGoogle Scholar
  10. Coordinating system for laboratory testing and surveillance [cited 2006 Apr 1]. Available from
  11. Yuen  KY, Chan  PK, Peiris  M, Tsang  DN, Que  TL, Shortridge  KF, Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus. Lancet. 1998;351:46771. DOIPubMedGoogle Scholar
  12. Spackman  E, Senne  DA, Myers  TJ, Perdue  ML, Garber  LP, Lohman  K, Development of a real-time reverse transcriptase PCR assay for type A influenza virus and the avian H5 and H7 hemagglutinin subtypes. J Clin Microbiol. 2002;40:325660. DOIPubMedGoogle Scholar
  13. Webster  R, Cox  N, Stohr  K. WHO manual on animal influenza diagnosis and surveillance. World Health Organization, Department of Communicable Disease Surveillance and Response. WHO/CDS/CDR/2002.5 Rev. 1.
  14. Rowe  T, Abernathy  RA, Hu-Primmer  J, Thompson  WW, Lu  X, Lim  W, Detection of antibody to avian influenza A (H5N1) virus in human serum by using a combination of serologic assays. J Clin Microbiol. 1999;37:93743.PubMedGoogle Scholar
  15. Apisarnthanarak  A, Kitphati  R, Tawatsupha  P, Thongphubeth  K, Apisarnthanarak  P, Mundy  LM. Varicella-zoster outbreak among Thai healthcare workers. Infect Control Hosp Epidemiol. 2006. In press.PubMedGoogle Scholar




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DOI: 10.3201/eid1211.060443

Table of Contents – Volume 12, Number 11—November 2006

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Anucha Apisarnthanarak, Division of Infectious Diseases, Department of Medicine, Thammasart University Hospital, Pratumthani, Thailand, 12000

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