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Volume 12, Number 11—November 2006
Letter

Real-time PCR for Francisella tularensis Types A and B

Kiersten J. Kugeler*, Ryan Pappert*, Yan Zhou*, and Jeannine M. Petersen*Comments to Author 
Author affiliations: *Centers for Disease Control and Prevention, Fort Collins, Colorado, USA

Main Article

Table

Comparison of standard diagnostic methods with the multitarget Francisella tularensis TaqMan assay and type A and type B assays using primary specimens

Specimen Source F. tularensis identified* Subspecies identification† Multitarget F. tularensis TaqMan assay‡
Type A assay‡
(Ct value)§ Type B assay‡
(Ct value)
ISFtu2 IglC tul4
Lymph node aspirate Human + + + + 31
Bronchial wash Human + A + + + 34
Upper lung Human + A + + + 20
Lower lung Human + A + + + 26
Liver Human + A + + + 29
Spleen Human + A + + + 31
Pleural fluid Human + B + + + 36
Blood Human + + + + 38
Spleen Human
Liver Human
Cerebrospinal fluid Human
Blood
Human







Liver/spleen Tamarin + + + + 28
Tissue Tick¶ + A + + + 26
Tissue Tick¶ + A + + + 33
Blood Prairie dog + B + + + 30
Blood Prairie dog + B + + + 27
Spleen Prairie dog + B + + + 21
Spleen Prairie dog + B + + + 31
Spleen Prairie dog
Liver Cat
Liver Rat
Spleen Rat
Spleen Squirrel

*F. tularensis infection identified by culture, direct fluorescent antibody testing, or serologic testing.
†Subspecies was determined by glycerol fermentation when an isolate was recovered.
‡+ = positive result, 17<Ct <38; – = negative result, no fluorescence detected after 45 cycles of amplification.
§Ct, cycle threshold.
¶Tick species tested were Haemaphysalis leporispalustris and Dermacentor andersoni.

*F. tularensis infection identified by culture, direct fluorescent antibody testing, or serologic testing.
†Subspecies was determined by glycerol fermentation when an isolate was recovered.
‡+ = positive result, 17<Ct <38; – = negative result, no fluorescence detected after 45 cycles of amplification.
§Ct, cycle threshold.
¶Tick species tested were Haemaphysalis leporispalustris and Dermacentor andersoni.

Main Article

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Page updated: October 14, 2011
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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