Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 12, Number 12—December 2006
Letter

Assay to Detect Oseltamivir Resistance

Kamol Suwannakarn*, Salin Chutinimitkul*, Sunchai Payungporn*, Thaweesak Chieochansin*, Apiradee Theamboonlers*, Alongkorn Amonsin*, Sudarat Damrongwatanapokin†, Le Quynh Mai‡, Nguyen Hong Hanh‡, and Yong Poovorawan*Comments to Author 
Author affiliations: *Chulalongkorn University, Bangkok, Thailand; †National Institute of Animal Health, Bangkok; ‡National Institute of Hygiene and Epidemiology, Hanoi, Vietnam

Main Article

Figure

A) Representative result from conventional PCR that used H274r primer for oseltamivir-sensitive and Y274r primer for oseltamivir-resistant detection in samples isolated from human plasma (P), tiger (T), and Vietnamese patient (V). Plasmids containing N1 fragments obtained from PCR-based mutagenesis for wild-type H274 (Wt) and mutant Y274 (Mt) were used as positive controls in each reaction. (N, no template control; M, 100-bp molecular marker.) B) Semiquantitative data on the ability of the assay

Figure. A) Representative result from conventional PCR that used H274r primer for oseltamivir-sensitive and Y274r primer for oseltamivir-resistant detection in samples isolated from human plasma (P), tiger (T), and Vietnamese patient (V). Plasmids containing N1 fragments obtained from PCR-based mutagenesis for wild-type H274 (Wt) and mutant Y274 (Mt) were used as positive controls in each reaction. (N, no template control; M, 100-bp molecular marker.) B) Semiquantitative data on the ability of the assay to detect subpopulations of the resistant variants. The 2 control plasmids were mixed at wild-type:variant ratios of 108:102 (lanes 1 and 8), 107:103 (lanes 2 and 9), 106:104 (lanes 3 and 10), 105:105 (lanes 4 and 11), 104:106 (lanes 5 and 12), 103:107 (lanes 6 and 13), and 102:108 (lanes 7 and 14).

Main Article

Page created: October 04, 2011
Page updated: October 04, 2011
Page reviewed: October 04, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external