Volume 12, Number 2—February 2006
Perspective
Detecting Hepatitis B Surface Antigen Mutants
Figure

Figure. Gly/Arg 145 mutant in the projecting amino acid 139–147 antigenic loop of the "a" determinant. This mutant produces false-negative results in some commercial assays. Image courtesy of Y.C. Chen et al. (11).
References
- Kao JH, Chen DS. Global control of hepatitis B virus. Lancet Infect Dis. 2002;2:395–403. DOIPubMedGoogle Scholar
- Esteban J, Martell M, Carman WF, Gomez J, Esteban J, Martell M, The impact of rapid evolution of the hepatitis viruses. In: Domingo E, Webster RG, Holland JI, editors. Origin and evolution of viruses. London: Academic Press; 1999. p. 345–65.
- Norder H, Couroucé A, Coursaget P, Echevarria J, Lee S, Mushahwar I, Genetic diversity of hepatitis B virus strains derived worldwide: genotypes, subgenotypes, and HBsAg subtypes. Intervirology. 2004;47:289–309. DOIPubMedGoogle Scholar
- Ngui S, Hallet R, Teo C. Natural and iatrogenic variation in hepatitis B virus. Rev Med Virol. 1999;9:183–209. DOIPubMedGoogle Scholar
- Weinberger K, Bauer T, Bohm S, Jilg W. High genetic variability of the group-specific a-determinant of hepatitis B virus surface antigen (HBsAg) and the corresponding fragment of the viral polymerase in chronic virus carriers lacking detectable HBsAg in serum. J Gen Virol. 2000;81:1165–74.PubMedGoogle Scholar
- Locarnini S. Hepatitis B viral resistance: mechanisms and diagnosis. J Hepatol. 2003;39:Suppl S124–32.
- Nainan O, Khristova M, Byun K, Xia G, Taylor P, Stevens C, Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection. J Med Virol. 2002;68:319–27. DOIPubMedGoogle Scholar
- Mangold C, Streeck R. Mutational analysis of the cysteine residues in the hepatitis B virus small envelope protein. J Virol. 1993;67:4588–97.PubMedGoogle Scholar
- Khan N, Guarnieri M, Ahn S, Jisu Li, Zhou Y, Bang G, Modulation of hepatitis B virus secretion by naturally occurring mutations in the S gene. J Virol. 2004;78:3262–70. DOIPubMedGoogle Scholar
- Qiu X, Schroeder P, Bridon D. Identification and characterization of a C(K/R)TC motif as a common epitope present in all subtypes of hepatitis B surface antigen. J Immunol. 1996;156:3350–6.PubMedGoogle Scholar
- Chen Y-C, Delbrook K, Dealwis C, Mimms L, Mushahwar I, Mandecki W. Discontinuous epitopes of hepatitis B surface antigen derived from a filamentous phage peptide library. Proc Natl Acad Sci U S A. 1996;93:1997–2001. DOIPubMedGoogle Scholar
- Antoni BA, Rodriguez-Crespo I, Gomez-Gutierrez J, Nieto M, Peterson D, Gavilanes F. Site-directed mutagenesis of cysteine residues of hepatitis B surface antigen. Eur J Biochem. 1994;222:121–7. DOIPubMedGoogle Scholar
- Maillard P, Pillot J. At least three epitopes are recognized by the human repertoire in the hepatitis B virus group a antigen inducing protection; possible consequences for seroprevention and serodiagnosis. Res Virol. 1998;149:153–61. DOIPubMedGoogle Scholar
- Shearera M, Sureaub C, Dunbarc B, Kennedy R. Structural characterization of viral neutralizing monoclonal antibodies to hepatitis B surface antigen. Mol Immunol. 1998;35:1149–60. DOIPubMedGoogle Scholar
- Jolivet-Reynaud C, Lesenchal M, O'Donnell B, Becquant L, Foussadiez A, Forge F, Localization of hepatitis B surface antigen epitopes present on variants and specifically recognized by anti-hepatitis B surface antigen monoclonal antibodies. J Med Virol. 2001;65:241–9. DOIPubMedGoogle Scholar
- Zanetti A, Tanzi E, Manzillo G, Maio G, Sbreglia C, Caporaso N, Hepatitis B variant in Europe. Lancet. 1988;2:1132–3. DOIPubMedGoogle Scholar
- Carman W, Zanetti A, Karayiannis P, Waters J, Manzillo G, Tanzi E, Vaccine-induced escape mutant of hepatitis B virus. Lancet. 1990;336:325–9. DOIPubMedGoogle Scholar
- Shizuma T, Hasegawa K, Ishikawa K, Naritomi T, Iizuka A, Kanai N, Molecular analysis of antigenicity and immunogenicity of a vaccine-induced escape mutant of hepatitis B virus. J Gastroenterol. 2003;38:244–53. DOIPubMedGoogle Scholar
- Ogata N, Zanetti AR, Yu M, Miller RH, Purcell RH. Infectivity and pathogenicity in chimpanzees of a surface gene mutant of hepatitis B virus that emerged in a vaccinated infant. J Infect Dis. 1997;175:511–23. DOIPubMedGoogle Scholar
- Ogata N, Cote PJ, Zanetti AR, Miller RH, Shapiro M, Gerin J, Licensed recombinant hepatitis B vaccines protect chimpanzees against infection with the prototype surface gene mutant of hepatitis B virus. Hepatology. 1999;30:779–86. DOIPubMedGoogle Scholar
- Wilson J, Nokes D, Carman W. Predictions of the emergence of vaccine-resistant hepatitis B in The Gambia using a mathematical model. Epidemiol Infect. 2000;124:295–307. DOIPubMedGoogle Scholar
- Zuckerman J, Zuckerman A. Mutations of the surface protein of hepatitis B virus. Antiviral Res. 2003;60:75–8. DOIPubMedGoogle Scholar
- Carman W. The clinical significance of surface antigen variants of hepatitis B virus. J Viral Hepat. 1997;4:11–20. DOIPubMedGoogle Scholar
- Yamamoto K, Horikita M, Tsuda F, Itoh K, Akahane Y, Yotsumoto S, Naturally occurring escape mutants of hepatitis B virus with various mutations in the S gene in carriers seropositive of antibody to hepatitis B surface antigen. J Virol. 1994;68:2671–6.PubMedGoogle Scholar
- Hou J, Karayiannis P, Waters J, Lou K, Liang C, Thomas H. A unique insertion in the S gene of surface antigen-negative hepatitis B virus Chinese carriers. Hepatology. 1995;21:273–8.PubMedGoogle Scholar
- Weinberger K, Zoulek G, Bauer T, Bohm S, Jilg W. A novel deletion mutant of hepatitis B virus surface antigen. J Med Virol. 1999;58:105–10. DOIPubMedGoogle Scholar
- Gunther S, Sommer G, von Breunig F, Iwanska A, Kalinina T, Sterneck M, Amplification of full-length hepatitis B virus genomes from samples from patients with low levels of viremia: frequency and functional consequences of PCR-introduced mutations. J Clin Microbiol. 1998;36:531–8.PubMedGoogle Scholar
- Carman W, van Deursen F, Mimms L, Hardie D, Coppola R, Decker R, The prevalence of surface antigen variants of hepatitis B virus in Papua New Guinea, South Africa, and Sardinia. Hepatology. 1997;26:1658–66. DOIPubMedGoogle Scholar
- Coleman P, Chen Y-C, Mushahwar IK. Immunoassay detection of hepatitis B surface antigen mutants. J Med Virol. 1999;59:19–24. DOIPubMedGoogle Scholar
- Ireland J, O'Donnell B, Basuni A, Kean J, Wallace L, Lau G, Reactivity of 13 in vitro expressed hepatitis B surface antigen variants in 7 commercial diagnostic assays. Hepatology. 2000;31:1176–82. DOIPubMedGoogle Scholar
- Moerman B, Moons V, Sommer H, Schmitt Y, Stetter M. Evaluation of sensitivity for wild-type and mutant forms of hepatitis B surface antigen by four commercial HBsAg assays. Clin Lab. 2004;50:159–62.PubMedGoogle Scholar
- Zaaijer HL, Vrielink H, Koot M. Early detection of hepatitis B surface antigen and detection of HBsAg mutants: a comparison of five assays. Vox Sang. 2001;81:219–21. DOIPubMedGoogle Scholar
- Levicnik-Stezinar S. Hepatitis B surface antigen escape mutant in a first time blood donor potentially missed by a routine screening assay. Clin Lab. 2004;50:49–51.PubMedGoogle Scholar
- Koyanagi T, Nakamuta M, Sakai H, Sugimoto R, Enjoji M, Koto K, Analysis of HBs antigen negative variant of hepatitis B virus: unique substitutions, Glu129 to Asp and Gly145 to Ala in the surface antigen gene. Med Sci Monit. 2000;6:1165–9.PubMedGoogle Scholar
- Tallo T, Norder H, Tefanova V, Krispin T, Priimagi L, Mukomolov S, Hepatitis B virus genotype D strains from Estonia share sequence similarity with strains from Siberia and may specify ayw4. J Med Virol. 2004;74:221–7. DOIPubMedGoogle Scholar
- Gerlich W. Diagnostic problems caused by HBsAg mutants—a consensus report of an expert meeting. Intervirology. 2004;47:310–3. DOIPubMedGoogle Scholar
- Oon C, Lim G, Ye Z, Goh K, Tan K, Yo S, Molecular epidemiology of hepatitis B virus vaccine variants in Singapore. Vaccine. 1995;13:699–702. DOIPubMedGoogle Scholar
- Hsu HY, Chang MH, Liaw SH, Ni YH, Chen HL. Changes of hepatitis B surface antigen variants in carrier children before and after universal vaccination in Taiwan. Hepatology. 1999;30:1312–7. DOIPubMedGoogle Scholar
- Thakur V,1 Kazim S, Guptan R,1 Hasnain S, Bartholomeusz A, Malhotra V, Transmission of G145R mutant of HBV to an unrelated contact. J Med Virol. 2005;76:40–6. DOIPubMedGoogle Scholar
- Torresi J, Earnest-Silveira L, Civitico G, Walters TE, Lewin SR, Fyfe J, Restoration of replication phenotype of lamivudine-resistant hepatitis B virus mutants by compensatory changes in the "fingers" subdomain of the viral polymerase selected as a consequence of mutations in the overlapping S gene. Virology. 2002;299:88–99. DOIPubMedGoogle Scholar