Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 12, Number 3—March 2006
Research

Identifying and Quantifying Genotypes in Polyclonal Infections due to Single Species

James M. Colborn*, Ousmane Koita†, Ousmane Cissé†, Mamadou W. Bagayoko†, Edward J. Guthrie‡, and Donald J. Krogstad*Comments to Author 
Author affiliations: *Tulane University Health Sciences Center, New Orleans, Louisiana, USA; †University of Bamako, Bamako, Mali; ‡Agilent Technologies, Wilmington, Delaware, USA

Main Article

Table 1

Primers and probes used for real-time polymerase chain reaction*

Primer/probe sequence Sizes (bp) Tm (°C) Fluorophore, quencher Final reactant concentration (nmol/L)
Parasites with K1 and hybrid sequences in block 2 of msp1
K1F 5´-AGGTGCAAGTGCTCAAAGTG-3´ 108–171 50.3 Texas Red, BHQ-2 100
K1R 5´-CACCAGATGAAGTATTTGAACG-3´ 49.2 100
PROBE: 5´-AAGTGGTACAAGTCCATCATCTCGT-3´ 54.9 300
Hybrid F 5´-GAAGGAACAAGTGGAACAGC-3´ 96–168 48.7 Cy5, BHQ-2 200
Hybrid R 5´-GCAGCACCTGGAGATCTTATA-3´ 48.8 200
PROBE: 5´-TTCACTTATTTCCCCATGAGCCCC-3´ 54.2 400
Primers for positive control (EBA175)
EBA175 F 5´-GGTTATTCAACTAAGGCAGAA-3´ 95 46.0 Cy 5, BHQ-3 100
EBA175 R 5´-TCCACCATTCTTTTCTAAAATTTT-3´ 50.6 100
PROBE: 5´-TCATTTCCCATAGCAAGATGTCC 60.0 100
LUX primers (MAD 20, RO33)
MAD20 F 5´-AATGAAGGAACAAGTGGAAC-3´ 52–205 48.7 FAM 200
MAD20 R 5´-GAATTATCTGAAGGATTTGTACG-3´ 47.4 (reverse) 200
RO33 F 5´-GCAGATGCTGTAAGTACTCAA-3´ 148 44.8 JOE 200
RO33 R 5´-GCAGCACCTGGAGATCTTATA-3´ 44.8 (forward) 200

*msp1, merozoite surface protein 1, EBA175, erythrocyte-binding antigen 175; Tm, melting temperature.

Main Article

Page created: January 27, 2012
Page updated: January 27, 2012
Page reviewed: January 27, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external