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Volume 12, Number 3—March 2006
Research

Aspergillus ustus Infections among Transplant Recipients

Anil A. Panackal*†, Alexander Imhof*‡, Edward W. Hanley*, and Kieren A. Marr*†Comments to Author 
Author affiliations: *Fred Hutchinson Cancer Research Center; Seattle, Washington, USA; †University of Washington Medical Center, Seattle, Washington, USA; ‡University Hospital, Zürich, Zürich, Switzerland

Main Article

Table 1

Conditions for Aspergillus ustus DNA amplification.

Primers
Ustus 1 R151 RPO2 OPA10 OPA20
Primer sequence 5´-GTA TTG CCC T-3´ 5´-GCT GTA GTG T-3´ 5´-GCG ATC CCC A-3´ 5´-GTG ATC GCA G-3´ 5´-GTT GCG ATC C-3´
Primer concentration 0.8 pmol/L 1.0 pmol/L 1.0 pmol/L 0.4 pmol/L 1.0 pmol/L
MgCl2 1.8 mmol/L 2.2 mmol/L 3.0 mmol/L 1.8 mmol/L 2.0 mmol/L
Template concentration 0.025 ng/50μL 0.5 ng/50μL 0.012 ng/50μL 0.03 ng/50μL 0.1 ng/50μL
Annealing temperature 32°C 32°C 34°C 32°C 32°C
Annealing time 1.5 min 1.5 min 1.5 min 1.5 min 1.5 min

Main Article

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