Volume 12, Number 3—March 2006
Research
Rickettsia prowazekii and Real-time Polymerase Chain Reaction
Sanela Svraka*, Jean-Marc Rolain*, Yassina Bechah*, John Gatabazi†, and Didier Raoult*
Table
Strains used in real-time PCR*
| Strains | Source | Standard PCR | LC PCR assay |
|---|---|---|---|
| Rickettsia prowazekii Breinl | ATCC | + | + |
| R. prowazekii Evir | UR | + | + |
| R. prowazekii BatnaRp22 | UR | + | + |
| R. prowazekii Russian sample | UR | + | + |
| R. typhi Wilmington | ATCC | + | – |
| R. massiliae MtuI | ATCC | + | – |
| R. montanensis | ATCC | + | – |
| "R. aeschlimanii" | UR | + | – |
| R. massiliae strain Bar 29 | UR | + | – |
| R. helvetica C6P9 | ATCC | + | – |
| R. felis | UR | + | – |
| "R. sibirica mongolitimonae" | UR | + | – |
| R. rickettsii | ATCC | + | – |
| R. conorii moroccan | ATCC | + | – |
| R. sibirica sibirica 246 | ATCC | + | – |
| R. conorii subsp. israelensis CDC1 | G.A. Dasch | + | – |
| R. africae ESF–5 | UR | + | – |
| R. japonica YM | ATCC | + | – |
| Thai tick typhus rickettsia | G.A. Dasch | + | – |
| R. slovaca | UR | + | – |
| R. conorii subsp. caspia A-167 | UR | + | – |
| R. australis Phillips | G.A. Dasch | + | – |
| R. honei RB | GRIC | + | – |
| Rickettsia sp. AT1 | UR | + | – |
| R. bellii 369L42-1 | D.H. Walker | + | – |
| 31 lice from Rwanda (2004) | J. Bosco | + (10/31)† | +(17/31)† |
| 30 lice from Rwanda (2005) | J. Gatabazi | ND | +(5/30) |
| R. prowazekii laboratory-infected lice | UR | + (10/10) | +(10/10) |
| R. typhi laboratory-infected lice | UR | + (10/10) | – |
| R. prowazekii–infected BALB/C mice | UR | + (12/12)‡ | + (12/12)‡ |
| B. recurrentis laboratory-infected lice | UR | – (0/10) | – (0/10) |
| Borrelia recurrentis | ATCC | – | – |
| Escherichia coli | CIP | – | – |
| Proteus mirabilis | CIP | – | – |
| Staphylococcus aureus | CIP | – | – |
| Streptococcus salivarius | CIP | – | – |
| Orientia tsutsugamushi | CIP | + | – |
| Streptococcus pyogenes | CIP | – | – |
| Mycobacterium xenopi | CIP | – | – |
| Chlamydia trachomatis | Human isolate | – | – |
| Propionibacterium acnes | UR | – | – |
| HGE agent | ATCC | – | – |
| Bartonella quintana Oklahoma | ATCC | – | – |
| Tropheryma whipplei Twist | UR | – | – |
| M. tuberculosis | CIP | – | – |
*PCR, polymerase chain reaction; LC, LightCycler; ATCC, American Type Culture Collection, Rockville, MD, USA; GRIC, Gamaleya Research Institute Collection; G.A. Dasch, Naval Medical Research Institute, Bethesda, MD, USA; UR, Unité des Rickettsies, CNRS UPRES A, Marseille, France; D.H. Walker, University of Texas, Galveston; CIP, Collection Institute Pasteur, Paris, France; HGE, human granulocytic ehrlichiosis.
†Number of positive lice/total number of tested lice.
‡PCR for each mouse was positive in blood at days 3 and 6 postinfection (for cycle thresholds see results in text).
Page created: January 27, 2012
Page updated: January 27, 2012
Page reviewed: January 27, 2012
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