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Volume 12, Number 7—July 2006

Replicon Typing of Plasmids Encoding Resistance to Newer β-Lactams

Alessandra Carattoli*Comments to Author , Vivi Miriagou†, Alessia Bertini*, Alexandra Loli†, Celine Colinon‡, Laura Villa*, Jean M. Whichard§, and Gian Maria Rossolini‡
Author affiliations: *Istituto Superiore di Sanità, Rome, Italy; †Institute Pasteur, Athens, Greece; ‡Università di Siena, Siena, Italy; §Centers for Disease Control and Prevention, Atlanta, Georgia, USA

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Figure. Restriction enzyme analysis of plasmids analyzed in this study. Numbers and letters above each lane indicate strain reference names as defined in the Table. M is the1-kb Plus DNA ladder (Gibco-BRL, Gaithersburg, MD, USA). Plasmids were transferred by conjugation or transformation in Escherichia coli K12 strains and purified by Midi-prep purification kit. Plasmids were digested with restriction endonuclease PstI and separated by agarose (1%) gel electrophoresis in 1× TBE buffer. DNA was stained with ethidium bromide and visualized under UV light. The replicons and the β-lactamase genes carried by each group of plasmids are indicated below the figure.

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