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Volume 12, Number 7—July 2006

Replicon Typing of Plasmids Encoding Resistance to Newer β-Lactams

Alessandra Carattoli*Comments to Author , Vivi Miriagou†, Alessia Bertini*, Alexandra Loli†, Celine Colinon‡, Laura Villa*, Jean M. Whichard§, and Gian Maria Rossolini‡
Author affiliations: *Istituto Superiore di Sanità, Rome, Italy; †Institute Pasteur, Athens, Greece; ‡Università di Siena, Siena, Italy; §Centers for Disease Control and Prevention, Atlanta, Georgia, USA

Main Article


Phenotypic and genetic characteristics of plasmids and transformant/transconjugant strains analyzed in this study

Original strain Species and serovar Transferred resistance traits in transconjugants or transformants* bla genes identified on transferred plasmids Replicons detected by PCR†
USA-4204 Salmonella enterica serovar Typhimurium AmpCazCroCtxFox CMY-2-type A A/C2
USA-2039 S. Typhimurium AmpCazCroCtxFoxGmTo CMY-2-type A A/C2
USA-3977 S. Typhimurium AmpCazCroCtxFox CMY-2-type A A/C2
USA-8401 Escherichia coli O157:H7 AmpCazCroCtxFox CMY-2-typeA A/C2
USA-8749 E. coli O157:H7 AmpCazCroCtxFox CMY-2-type A A/C2
USA-8868 E. coli O157:H7 AmpCazCroCtxFox CMY-2-type A A/C2
USA-1091 E. coli O157:H7 AmpCazCroCtxFox CMY-2-type A A/C2
USA-7546 E. coli O157:H7 AmpCazCroCtxFox CMY-2-type A A/C2
USA-11371 E. coli O157:H7 AmpAtmCazCroCtxFox CMY-2-type B I1
USA-1358 S. Thompson AmpAtmCazCroCtxFox CMY-2-type B I1
IT-VA416/02 Klebsiella pneumoniae AmpAtmCazCroCtxFoxIpmGmTo VIM-4, CMY-4 A/C2
IT-VA417/02 Enterobacter cloacae AmpAtmCazCroCtxFoxIpmGmTo VIM-4, CMY-4 A/C2
IT-FI045T Enterobacter aerogenes AmpAtmCaz SHV-12 FII
IT-FI008T E. coli AmpAtmCazTo SHV-12 FII
IT-BG003T Serratia marcescens AmpAtmCazTo SHV-12 FII
IT-NO003T Klebsiella oxytoca AmpAtmCaz SHV-12 A/C1
IT-BG017T K. pneumoniae AmpAtmCazCroCtx SHV-12 I1
GR-541 E. coli AmpAtmCazCtxCroFoxIpmTo VIM-1, CMY13 N
GR-116 E. coli AmpAtmCazCroCtxFoxIpmGmTo VIM-1, CMY13 N
GR-700 K. pneumoniae AmpCazCroCtxFoxIpmGmTo VIM-1 N
GR-2564 K. pneumoniae AmpCazCroCtxFoxIpmTo VIM-1 N
GR-1943 K. pneumoniae AmpCazCroCtxFoxIpmTo VIM-1 N
GR-1955 K. pneumoniae AmpCazCroCtxFoxIpmTo VIM-1 N
GR-5866 K. pneumoniae AmpCazCroCtxFoxIpmTo VIM-1 N
GR-51395 K. pneumoniae AmpCazCroCtxFoxIpmTo VIM-1 N
GR-6/100 K. pneumoniae AmpCazCroCtxFoxIpmTo VIM-1 N

*Transconjugants or transformants were obtained by conjugation on solid medium or electroporation, respectively, with E. coli (strains DH5-α or DH10B or 26R793 as recipients), as described previously (510). Antimicrobial drug susceptibility of the transconjugants or transformants was determined by disk diffusion as recommended by the Committee on Clinical and Laboratory Standards (11) with the following drugs: Amp, ampicillin; Atm, aztreonam; Caz, ceftazidime; Cro, ceftriaxone; Ctx, cefotaxime; Fox, cefoxitin; Ipm, imipenem; Gm, gentamicin; To, tobramycin. Transferred resistance traits were inferred by a detectable reduction of susceptibility to the drug in the transconjugant or transformant, compared to the E. coli recipient.
†Discrimination between A/C1 and A/C2 replicons was performed by DNA sequencing of the amplicon obtained by replicon typing.

Main Article

  1. Novick  RP. Plasmid incompatibility. Microbiol Rev. 1987;51:38195.PubMedGoogle Scholar
  2. Datta  N, Hughes  VM. Plasmids of the same Inc groups in Enterobacteria before and after the medical use of antibiotics. Nature. 1983;306:6167. DOIPubMedGoogle Scholar
  3. Couturier  M, Bex  F, Bergquist  PL, Maas  WK. Identification and classification of bacterial plasmids. Microbiol Rev. 1988;52:37595.PubMedGoogle Scholar
  4. Carattoli  A, Bertini  A, Villa  L, Falbo  V, Hopkins  KL, Threlfall  EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005;63:21928. DOIPubMedGoogle Scholar
  5. Whichard  JM, Joyce  K, Fey  P, Nelson  JM, Angulo  FJ, Barrett  TJ. β-lactam resistance and Enterobacteriaceae, United States. Emerg Infect Dis. 2005;11:14646.PubMedGoogle Scholar
  6. Carattoli  A, Tosini  F, Giles  WP, Rupp  ME, Hinrichs  SH, Angulo  FJ, Characterization of plasmids carrying CMY-2 from expanded-spectrum cephalosporin-resistant Salmonella isolated in the United States between 1996 and 1998. Antimicrob Agents Chemother. 2002;46:126972. DOIPubMedGoogle Scholar
  7. Luzzaro  F, Docquier  JD, Colinon  C, Endimiani  A, Lombardi  G, Amicosante  G, Emergence in Klebsiella pneumoniae and Enterobacter cloacae clinical isolates of the VIM-4 metallo-β-lactamase encoded by a conjugative plasmid. Antimicrob Agents Chemother. 2004;48:64850. DOIPubMedGoogle Scholar
  8. Luzzaro  F, Mezzatesta  M, Mugnaioli  C, Perilli  M, Stefani  S, Amicosante  G, Trends in the production of extended-spectrum β-lactamases among enterobacteria of medical interest. Report of the second Italian nationwide survey. J Clin Microbiol. 2006;44:165964. DOIPubMedGoogle Scholar
  9. Giakkoupi  P, Xanthaki  A, Kanelopoulou  M, Vlahaki  A, Miriagou  V, Kontou  S, VIM-1 metallo-β-lactamase-producing Klebsiella pneumoniae strains in Greek Hospitals. J Clin Microbiol. 2003;41:38936. DOIPubMedGoogle Scholar
  10. Miriagou  V, Tzelepi  E, Gianneli  D, Tzouvelekis  LS. Escherichia coli with a self-transferable, multi-resistant plasmid coding for the metallo-β-lactamase VIM-1. Antimicrob Agents Chemother. 2003;47:3957. DOIPubMedGoogle Scholar
  11. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk susceptibility tests; approved standard. 9th ed. Wayne (PA): The Institute; 2006.
  12. Dunne  EF, Fey  PD, Kludt  P, Reporter  R, Mostashari  F, Shillam  P, Emergence of domestically acquired ceftriaxone-resistant Salmonella infections associated with AmpC beta-lactamase. JAMA. 2000;284:31516. DOIPubMedGoogle Scholar
  13. Giles  WP, Benson  AK, Olson  ME, Hutkins  RW, Whichard  JM, Winokur  PL, DNA sequence analysis of regions surrounding blaCMY-2 from multiple Salmonella plasmid backbones. Antimicrob Agents Chemother. 2004;48:284552. DOIPubMedGoogle Scholar
  14. Miriagou  V, Carattoli  A, Tzelepi  E, Villa  L, Tzouvelekis  LS. IS26-associated In4-type integrons forming multiresistance loci in enterobacterial plasmids. Antimicrob Agents Chemother. 2005;49:35413. DOIPubMedGoogle Scholar
  15. Perilli  M, Dell'Amico  E, Segatore  B, De Massis  MR, Bianchi  C, Luzzaro  F, Molecular characterization of extended-spectrum β-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey. J Clin Microbiol. 2002;40:6114. DOIPubMedGoogle Scholar

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Page updated: December 19, 2011
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