Figure 2. A) Phylogenetic tree of the 19 rabid skunk isolates and representative samples of known rabies virus variants (RABVV) from Arizona based on 300 bp of the nucleoprotein (N) gene (GenBank accession no. AY170226–304). B) Detailed analyses of clade including all 19 skunk isolates (clade B) based on 2221 bp of the N and glycoprotein (G) genes (GenBank accession no. AY170397–438). Phylogenetic analyses used PAUP* software (version 4.0b2, Sinauer Associates, Sunderland, MA, USA; 2000] using the neighbor-joining search algorithm (minimum evolution) with maximum likelihood to estimate Ti:Tv ratio and nucleotide base frequencies (HKY85 model). Numbers at tree nodes indicate nonparametric bootstrap proportions based on 1,000 replicates.