Volume 13, Number 11—November 2007
Protection and Virus Shedding of Falcons Vaccinated against Highly Pathogenic Avian Influenza A Virus (H5N1)
|Falcon no.,vaccination route||RNA in tissue, ct value†
*Viral RNA detected by real-time reverse transcription–PCR (RT-PCR) in vaccinated falcons euthanized 11 days postchallenge and in nonvaccinated falcons that died after challenge infection with 106.0 50% egg infectious doses of highly pathogenic avian influenza virus strain A/Cygnus cygnus/Germany/R65/06 (H5N1). Ct, cycle of threshold; CNS, central nervous system; IM, intramuscular; SC, subcutaneous; IH, inhibited (samples extracted twice with the QIAGEN [Hilden, Germany] Viral RNA kit or Trizol [Invitrogen, Carlsbad, CA, USA]).
†Real-time RT-PCR results are presented as ct values. Ct values >40 are scored as negative.
‡Virus isolation attempted in embryonated chicken eggs.
§Virus was isolated from individual tissue sample.