Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 13, Number 11—November 2007

Protection and Virus Shedding of Falcons Vaccinated against Highly Pathogenic Avian Influenza A Virus (H5N1)

Michael Lierz*Comments to Author , Hafez M. Hafez*, Robert Klopfleisch†, Dörte Lüschow*, Christine Prusas*, Jens P. Teifke†, Miriam Rudolf†, Christian Grund†, Donata Kalthoff†, Thomas C. Mettenleiter†, Martin Beer†, and Timm Harder†
Author affiliations: *Freie Universität, Berlin, Germany; †Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany;

Main Article

Table 3

Viral RNA in tissues of 5 vaccinated and 5 nonvaccinated falcons*

Falcon no.,vaccination route RNA in tissue, ct value†
CNS Duodenum Pancreas Trachea Lung
1, IM 36.12‡ >40.00 38.84‡ >40.00 >40.00
2, IM 38.65‡ >40.00 >40.00 >40.00 >40.00
5, IM 34.22‡ 36.14‡ >40.00 33.44‡§ 36.94‡
8, SC >40.00 IH >40.00 38.71‡ 32.85‡§
9, SC >40.00 IH IH 29.01§ IH
11 18.22 25.84 19.70 19.09 20.75
12 23.79 23.37 14.67 19.07 18.95
13 12.85 26.22 20.13 19.82 16.95
14 11.52 21.66 17.14 16.70 15.55
15 14.13 16.31 19.91 14.61 18.04

*Viral RNA detected by real-time reverse transcription–PCR (RT-PCR) in vaccinated falcons euthanized 11 days postchallenge and in nonvaccinated falcons that died after challenge infection with 106.0 50% egg infectious doses of highly pathogenic avian influenza virus strain A/Cygnus cygnus/Germany/R65/06 (H5N1). Ct, cycle of threshold; CNS, central nervous system; IM, intramuscular; SC, subcutaneous; IH, inhibited (samples extracted twice with the QIAGEN [Hilden, Germany] Viral RNA kit or Trizol [Invitrogen, Carlsbad, CA, USA]).
†Real-time RT-PCR results are presented as ct values. Ct values >40 are scored as negative.
‡Virus isolation attempted in embryonated chicken eggs.
§Virus was isolated from individual tissue sample.

Main Article

Page created: July 06, 2010
Page updated: July 06, 2010
Page reviewed: July 06, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.