Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 13, Number 12—December 2007
Dispatch

Parachlamydia spp. and Related Chlamydia-like Organisms and Bovine Abortion

Nicole Borel*, Silke Ruhl*, Nicola Casson†, Carmen Kaiser*, Andreas Pospischil*, and Gilbert Greub†Comments to Author 
Author affiliations: *University of Zurich, Zurich, Switzerland; †University of Lausanne, Lausanne, Switzerland;

Main Article

Table

Results of histologic, 16S rRNA sequence, and immunohistochemical analyses for 43 placentas positive for Chlamydia-like DNA by a 16S rRNA PCR*

Specimen no. Histology
16S rRNA sequence†
Immunohistochemistry
Placentitis Vasculitis Species % Similarity Parachlamydia spp. Waddlia
1 N Yes Parachlamydia 99 +
2 N No Parachlamydia 97 +
3 P/N No Parachlamydia 98 +
4 P/N No Parachlamydia 97
5 P/N No Parachlamydia 97
6 A No Parachlamydia 96 +
7 A No Parachlamydia 96 +
8 A No Parachlamydia 97 +
9 P/N Yes Chlamydia-like 92
10 P/N Yes Chlamydia-like 92
11 P/N Yes Chlamydia-like 93
12 P/N Yes Chlamydia-like 91 +
13 P/N No Chlamydia-like 82 +
14 P/N No Chlamydia-like 91 +
15 P/N No Chlamydia-like 92 +
16 P/N No Chlamydia-like 92 +
17 P/N No Chlamydia-like 92 +
18 P/N No Chlamydia-like 92 +
19 P/N No Chlamydia-like 92 +
20 P/N No Chlamydia-like 92 +
21 P/N No Chlamydia-like 93 +
22 P/N No Chlamydia-like 94 +
23 P/N No Chlamydia-like 95 +
24 P/N No Chlamydia-like 100 +
25 P/N No Chlamydia-like 93
26 P/N No Chlamydia-like 93
27 P/N No Chlamydia-like 95
28 P/N No Chlamydia-like 96
29 N No Chlamydia-like 85 +
30 N No Chlamydia-like 88 +
31 N No Chlamydia-like 88 +
32 N No Chlamydia-like 91 +
33 N No Chlamydia-like 91 +
34 N No Chlamydia-like 95 +
35 P No Chlamydia-like 91 +
36 P No Chlamydia-like 94 +
37 A No Chlamydia-like 91 +
38 A No Chlamydia-like 92 +
39 A No Chlamydia-like 92 +
40 A No Chlamydia-like 91
41 A No Chlamydia-like 92
42 A No Chlamydia-like 93
43 A No Chlamydia-like 95

*When partial 16S rRNA sequence showed a similarity >95% with a recognized species (i.e., Parachlamydia acanthamoebae), the corresponding genus was reported (i.e., Parachlamydia spp.). Conversely, when the sequence showed a best BLAST (www.ncbi.nlm.nih.gov) hit with uncultured or uncharacterized Chlamydia-related organisms, the sequence was designated as being similar to a Chlamydia-like organism. N, necrotizing; +, positive; –, negative; P, purulent; A, autolysis.
†A 278-bp fragment was amplified and sequenced (9).

Main Article

References
  1. Aitken  ID, Clarkson  MJ, Linklater  K. Enzootic abortion of ewes. Vet Rec. 1990;126:1368.PubMedGoogle Scholar
  2. Pospischil  A, Thoma  R, Hilbe  M, Grest  P, Gebbers  JO. Abortion in woman caused by caprine Chlamydophila abortus (Chlamydia psittaci serovar 1). Swiss Med Wkly. 2002;132:646.PubMedGoogle Scholar
  3. Longbottom  D, Coulter  LJ. Animal chlamydioses and zoonotic implications. J Comp Pathol. 2003;128:21744. DOIPubMedGoogle Scholar
  4. Friedman  MG, Dvoskin  B, Kahane  S. Infections with the Chlamydia-like microorganism Simkania negevensis, a possible emerging pathogen. Microbes Infect. 2003;5:101321. DOIPubMedGoogle Scholar
  5. Corsaro  D, Greub  G. Pathogenic potential of novel chlamydiae and diagnostic approaches to infections due to these obligate intracellular bacteria. Clin Microbiol Rev. 2006;19:28397. DOIPubMedGoogle Scholar
  6. Greub  G, Raoult  D. Parachlamydiaceae: potential emerging pathogens. Emerg Infect Dis. 2002;8:62530.PubMedGoogle Scholar
  7. Dilbeck  PM, Evermann  JF, Crawford  TB, Ward  AC, Leathers  CW, Holland  CJ, Isolation of a previously undescribed rickettsia from an aborted bovine fetus. J Clin Microbiol. 1990;28:8146.PubMedGoogle Scholar
  8. Henning  K, Schares  G, Granzow  H, Polster  U, Hartmann  M, Hotzel  H, Neospora caninum and Waddlia chondrophila strain 2032/99 in a septic stillborn calf. Vet Microbiol. 2002;85:28592. DOIPubMedGoogle Scholar
  9. Borel  N, Thoma  R, Spaeni  P, Weilenmann  R, Teankum  K, Brugnera  E, Chlamydia-related abortions in cattle from Graubunden, Switzerland. Vet Pathol. 2006;43:7028. DOIPubMedGoogle Scholar
  10. Casson  N, Entenza  JM, Greub  G. Serological cross-reactivity between different Chlamydia-like organisms. J Clin Microbiol. 2007;45:2346. DOIPubMedGoogle Scholar
  11. Borel  N, Mukhopadhyay  S, Kaiser  C, Sullivan  ED, Miller  RD, Timms  P, Tissue MicroArray (TMA) analysis of normal and persistent Chlamydophila pneumoniae infection. BMC Infect Dis. 2006;6:152. DOIPubMedGoogle Scholar
  12. Greub  G, Raoult  D. Crescent bodies of Parachlamydia acanthamoeba and its life cycle within Acanthamoeba polyphaga: an electron micrograph study. Appl Environ Microbiol. 2002;68:307684. DOIPubMedGoogle Scholar
  13. Casson  N, Medico  N, Bille  J, Greub  G. Parachlamydia acanthamoebae enters and multiplies within pneumocytes and lung fibroblasts. Microbes Infect. 2006;8:1294300. DOIPubMedGoogle Scholar
  14. Greub  G, Mege  JL, Raoult  D. Parachlamydia acanthamoebae enters and multiplies within human macrophages and induces their apoptosis. Infect Immun. 2003;71:597985. DOIPubMedGoogle Scholar

Main Article

Page created: July 06, 2010
Page updated: July 06, 2010
Page reviewed: July 06, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external