Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 13, Number 3—March 2007
Research

Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1

Stephen Mark Tompkins*1Comments to Author , Zi-Shan Zhao*, Chia-Yun Lo*, Julia A. Misplon*, Teresa Liu*, Zhiping Ye*, Robert J. Hogan†, Zhengqi Wu*, Kimberly A. Benton*, Terrence M. Tumpey‡, and Suzanne L. Epstein*
Author affiliations: *Food and Drug Administration, Bethesda, Maryland, USA; †University of Georgia, Athens, Georgia, USA; ‡Centers for Disease Control and Prevention, Atlanta, Georgia, USA;

Main Article

Figure 6

Role of T- and B-cell immunity in matrix protein 2 (M2)–specific protective immunity. A) Mice (9 per group) were immunized with M2-DNA or B/NP-DNA and boosted with matched adenovirus (Ad) as described in Methods. Three weeks after Ad boost, M2-DNA groups were acutely depleted of T cells with monoclonal antibodies (MAbs) to CD4+ or CD8+ or both, or given control MAb SFR3-DR5, as described in Methods. Mice were challenged with 1.5× 104 50% lethal doses (LD50) of A/PR/8. Compared with the cumulativ

Figure 6. Role of T- and B-cell immunity in matrix protein 2 (M2)–specific protective immunity. A) Mice (9 per group) were immunized with M2-DNA or B/NP-DNA and boosted with matched adenovirus (Ad) as described in Methods. Three weeks after Ad boost, M2-DNA groups were acutely depleted of T cells with monoclonal antibodies (MAbs) to CD4+ or CD8+ or both, or given control MAb SFR3-DR5, as described in Methods. Mice were challenged with 1.5× 104 50% lethal doses (LD50) of A/PR/8. Compared with the cumulative survival rate for the SFR control, survival rates differed significantly for mice depleted of both T-cell subsets (p<0.001, log-rank), although some protection remained, which differed significantly from that of the B/NP control (p<0.001, log-rank). B) Mice were immunized with M2-DNA+M2-Ad as described under A. Five months after mice received the Ad boost, spleen cells were isolated and pooled from immune mice (n = 10), fractionated into T-cell and non–T-cell populations, and assayed for interferon-γ (IFN-γ)–producing cells by enzyme-linked immunosorbent spot assay, as described in Methods. C and D) Serum collected from immune mice was passively transferred intraperitoneally into naive BALB/c mice (8 per group). The recipients were challenged with 10 LD50 of A/PR/8 and monitored for survival (C) and weight loss (D). The cumulative survival rate for mice given A/PR/8 immune serum, M2-DNA+M2-Ad-immune serum, or M2e-H5(HK)/keyhole limpet hemocyanin–immune serum was significantly higher than that for mice given B/NP-DNA+B/NP-Ad–immune serum (p<0.001, log rank). For weight loss, M2 prime-boost differed from B/NP prime-boost at days 8, 10, and 13 (p≤0.003, analysis of variance; p<0.05, Holm-Sidak pairwise multiple comparison).

Main Article

1Current affiliation: University of Georgia, Athens, Georgia, USA

Page created: June 29, 2010
Page updated: June 29, 2010
Page reviewed: June 29, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external