Volume 13, Number 3—March 2007
Dispatch
Mouse-to-Human Transmission of Variant Lymphocytic Choriomeningitis Virus
Sébastien Emonet*, Karine Retornaz†, Jean-Paul Gonzalez‡, Xavier de Lamballerie*§, and Rémi N. Charrel*‡
Table
Detection of LCMV in mice trapped at patient’s home and in patient CSF*
| Sample | System 1 | System 2 | System 3 | Virus isolation | Interpreted result† | |
|---|---|---|---|---|---|---|
| First-round PCR | Second-round PCR | |||||
| Human | ||||||
| CSF 1 | – | + | + | NT | NT‡ | + |
| CSF 2 | – | + | + | NT | NT | + |
| Mouse | ||||||
| 1 | – | + | + | + | – | + |
| 2 | – | – | + | + | + | + |
| 3 | – | – | – | – | – | – |
| 4 | – | + | + | – | + | + |
| 5 | – | + | + | – | + | + |
| 6 | – | + | + | – | + | + |
| 7 | – | + | + | + | – | + |
| 8 | + | + | + | + | – | + |
| 9 | + | + | + | + | + | + |
| 10 | – | – | + | + | + | + |
| 11 | – | + | + | + | – | + |
| 12§ | – | + | + | + | + | + |
| 13 | – | + | + | + | + | + |
| 14 | – | – | – | – | – | – |
| 15 | – | – | + | + | + | + |
| 16 | – | + | + | + | + | + |
| 17 | – | + | + | + | – | + |
| 18 | + | + | + | + | + | + |
| 19 | – | – | + | – | + | + |
| 20 | – | + | + | + | + | + |
*LCMV, lymphocytic choriomeningitis virus; CSF, cerebrospinal fluid, NT, not tested.
†An animal was considered infected with LCMV if virus was isolated either in Vero cells or results of at least 2 of 3 PCRs were positive.
‡After diagnostic tests were performed from CSF, no material was available to attempt virus isolation.
§Virus strain characterized by full-length genome sequencing (GenBank accession nos. DQ286931 and DQ286932 for S and L RNA, respectively).
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