Volume 13, Number 8—August 2007
Dispatch
Babesia sp. EU1 from Roe Deer and Transmission within Ixodes ricinus
Table 2
PCR detection of Babesia sp. EU1 in blood samples from roe deer and in Ixodes ricinus larvae hatched from engorged females collected from roe deer*
Roe deer Identification | Primer BAB |
Primer EU1 |
||
---|---|---|---|---|
Cultures | Cultures | Larvae | ||
C105 | + | + | ||
C107 | + | + | L107.1 (+) | |
L107.2 (–) | ||||
C109 | + | + | L109 (+) | |
C110 | + | + | L110.2 (–) | |
C112 | + | + | L112.1 (–) | |
L112.2 (–) | ||||
C115 | + | – | ||
C117 | + | – | ||
C123 | + | – | ||
C128 | + | – | L128.3(+) | |
C129 | + | + | ||
C139 | + | + | ||
C151 | + | + | L151 (+) | |
C156 | + | + | ||
C162 | + | + | ||
C163 | + | + | ||
C164 | + | – | L164 (–) | |
C167 | + | + | ||
C171 | + | + | L171.1 (–) | |
C172 | + | – | ||
C176 | + | – | L176.7 (–) | |
C177 | + | – | L177.1 (–) | |
L177.2 (–) | ||||
L177.3 (+) | ||||
C179 | + | – | L179.1 (+) | |
C180 | + | + | ||
C185 | + | – | ||
C188 | + | – | ||
C193 | + | – | ||
C169 | + | + | ||
C189 | + | + | ||
C157 | + | + | ||
Total positive (%) | 29 | 17 (58.62) | 6/15 (40) |
*+, positive amplification of a product of the expected size; –, no amplification.