Figure 1. Western blot analyses. A) Molecular weight and frequency of IgG reactivity of Waddlia proteins, as determined by Western blots. B) and C) Four representative Western blot patterns of Waddlia IgG positive sera from recurrent and sporadic miscarriage groups. D) and E) Representative Western blot pattern of positive control (Waddlia hyper-immune mouse and rabbit serum, respectively). F) Western blot performed with Waddlia IgG positive sera, taken from patients who had miscarried before (b) and after (a) adsorption with Waddlia antigen. MW, molecular weight; nb, number.