Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 14, Number 1—January 2008

Streptococcus suis in Humans, Thailand

Daisuke Takamatsu*, Korawan Wongsawan†, Makoto Osaki*, Hiroto Nishino‡, Tomono Ishiji§, Prasit Tharavichitkul†, Banyong Khantawa†, Achara Fongcom¶, Shinji Takai#, and Tsutomu Sekizaki***Comments to Author 
Author affiliations: *National Institute of Animal Health, Tsukuba, Ibaraki, Japan; †Chiang Mai University, Chiang Mai, Thailand; ‡Northern District Livestock Health and Hygiene Office, Mito, Ibaraki, Japan; §Seibu Livestock Hygiene Service Center, Tonami, Toyama, Japan; ¶Lamphun Provincial Hospital, Lamphun, Thailand; #Kitasato University, Towada, Aomori, Japan; **Gifu University, Gifu, Gifu, Japan;

Main Article


Epidemiologic data of Streptococcus suis isolates from patients in Thailand, 1998–2002*

Isolate no.† Year of isolation Site of isolation Virulence-associated genes‡§ Serotype Diseases and symptoms ST (ST complex)
MNCM01 2000 Blood cps2J+/sly+/epf+/mrp+ Endocarditis 1 (1)
MNCM06 2000 Blood, CSF cps2J+/sly+/epf+/mrp+ 2 Neck stiffness, deafness (meningitis) 1 (1)
MNCM16 2000 CSF cps2J+/sly+/epf+/mrp+ 2 Neck stiffness (meningitis) 1 (1)
MNCM07 2000 Blood, CSF cps1J+/sly+/epf*/mrpS+ 14 Neck stiffness (meningitis), death 11 (1)
MNCM04 2000 Blood cps2J+/sly–/epf–/mrp**+ 2 Neck stiffness, deafness (meningitis) 25 (27)#
MNCM10 2000 Blood cps2J+/sly–/epf–/mrp**+ 2 Septicemia 25 (27)#
MNCM24 2001 Blood cps2J+/sly–/epf–/mrp**+ Endocarditis 25 (27)#
MNCM26 2001 Blood cps2J+/sly–/epf–/mrp**+ 2 Endocarditis, deafness (meningitis) 25 (27)#
MNCM51 2002 Blood cps2J+/sly–/epf–/mrp**+ 2 Septicemia, diarrhea, death 25 (27)#
MNCM55 2002 Blood cps2J+/sly–/epf–/mrp**+ 2 Septic shock, death 25 (27)#
LPH4 2001 Blood cps2J+/sly–/epf–/mrp**+ 2 Septicemia, diarrhea 25 (27)#
LPH12 2002 Blood cps2J+/sly–/epf–/mrp**+ 2 Septic shock, death 25 (27)#
MNCM43 2002 Blood cps2J+/sly–/epf–/mrp+ 2 Endocarditis 28 (27)
MNCM21 1998 CSF cps2J+/sly+/epf–/mrp– 2 Meningitis 101 (27)#
MNCM25 2001 Blood cps2J+/sly–/epf–/mrp**+ 2 Neck stiffness (meningitis), diarrhea, death 102 (27)#
MNCM54 2002 Blood cps2J+/sly–/epf–/–mrp**+ 2 Neck stiffness (meningitis), diarrhea 102 (27)#
MNCM33 2002 Blood, CSF cps2J+/sly–/epf–/mrp**+ 2 Neck stiffness (meningitis) 103 (27)#
LPH3 2001 Blood cps2J+/sly–/epf–/mrp**+ 2 Meningitis 103 (27)#
LPH5 2001 Blood cps2J+/sly–/epf–/mrp**+ 2 Septicemia 103 (27)#
MNCM50 2002 Blood cps2J+/sly+/epf–/mrp– 2 Pulmonary edema, death 104 (27)#

*ST, sequence type; CSF, cerebrospinal fluid.
†Isolates with MNCM number and LPH number were isolated from patients at Maharaj Nakorn Chiang Mai Hospital and Lamphun Hospital, Thailand, respectively.
‡Virulence-associated gene profiling was done as described previously (10). cps1J and cps2J, serotype 1 (and 14) and 2 (and 1/2) specific genes, respectively, involved in the capsular biosynthesis; sly, suilysin gene; epf, extracellular factor gene; mrp, muraminidase-released protein gene; +, positive; –, negative.
§epf*, an epf variant that produces an ≈3,000-bp fragment by PCR with primers described previously (10); mrp** and mrpS, mrp variants that produce ≈1,800-bp and ≈750-bp fragments, respectively, by PCR with primers described previously (10).
¶Coagglutination reaction using anti-serotype 2 serum was weak.
#ST25, ST101, ST102, ST103, and ST104 belong to the ST27 complex, only with a less-stringent approach that defines an ST complex by sharing of alleles at >5 of the 7 loci.

Main Article

  1. Staats  JJ, Feder  I, Okwumabua  O, Chengappa  MM. Streptococcus suis: past and present. Vet Res Commun. 1997;21:381407. DOIPubMedGoogle Scholar
  2. Vecht  U, Wisselink  HJ, van Dijk  JE, Smith  HE. Virulence of Streptococcus suis type 2 strains in newborn germfree pigs depends on phenotype. Infect Immun. 1992;60:5506.PubMedGoogle Scholar
  3. Jacobs  AAC, Loeffen  PLW, van den Berg  AJG, Storm  PK. Identification, purification, and characterization of a thiol-activated hemolysin (suilysin) of Streptococcus suis. Infect Immun. 1994;62:17428.PubMedGoogle Scholar
  4. Smith  HE, Damman  M, van der Velde  J, Wagenaar  F, Wisselink  HJ, Stockhofe-Zurwieden  N, Identification and characterization of the cps locus of Streptococcus suis serotype 2: the capsule protects against phagocytosis and is an important virulence factor. Infect Immun. 1999;67:17506.PubMedGoogle Scholar
  5. Vecht  U, Wisselink  HJ, Jellema  ML, Smith  HE. Identification of two proteins associated with virulence of Streptococcus suis type 2. Infect Immun. 1991;59:315662.PubMedGoogle Scholar
  6. Gottschalk  M, Lebrun  A, Wisselink  H, Dubreuil  JD, Smith  H, Vecht  U. Production of virulence-related proteins by Canadian strains of Streptococcus suis capsular type 2. Can J Vet Res. 1998;62:759.PubMedGoogle Scholar
  7. King  SJ, Leigh  JA, Heath  PJ, Luque  I, Tarradas  C, Dowson  CG, Development of a multilocus sequence typing scheme for the pig pathogen Streptococcus suis: identification of virulent clones and potential capsular serotype exchange. J Clin Microbiol. 2002;40:367180. DOIPubMedGoogle Scholar
  8. Chang  B, Wada  A, Ikebe  T, Ohnishi  M, Mita  K, Endo  M, Characteritics of Streptococcus suis isolated from patients in Japan. Jpn J Infect Dis. 2006;59:3979.PubMedGoogle Scholar
  9. Ye  C, Zhu  X, Jing  H, Du  H, Segura  M, Zheng  H, Streptococcus suis sequence type 7 outbreak, Sichuan, China. Emerg Infect Dis. 2006;12:12038.PubMedGoogle Scholar
  10. Silva  LMG, Baums  CG, Rehm  T, Wisselink  HJ, Goethe  R, Valentin-Weigand  P. Virulence-associated gene profiling of Streptococcus suis isolates by PCR. Vet Microbiol. 2006;115:11727. DOIPubMedGoogle Scholar

Main Article

Page created: July 08, 2010
Page updated: July 08, 2010
Page reviewed: July 08, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.