Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 14, Number 12—December 2008

Mycobacterium bovis Strains Causing Smear-Positive Human Tuberculosis, Southwest Ireland

Olabisi Ojo, Stella Sheehan, G. Daniel Corcoran, Melissa Okker, Karen Gover, Vladyslav Nikolayevsky, Timothy Brown, James Dale, Stephen V. Gordon1, Francis Drobniewski, and Michael B. PrenticeComments to Author 
Author affiliations: University College Cork, Cork, Ireland (O. Ojo, M.B. Prentice); Cork University Hospital, Cork (S. Sheehan, G.D. Corcoran, M.B. Prentice); Veterinary Laboratories Agency Weybridge, Surrey, UK (M. Okker, K. Gover, J. Dale, S.V. Gordon); Health Protection Agency Barts and the London School of Medicine, London, UK (V. Nikolayevsky, T. Brown, F. Drobniewski);

Main Article

Table 2

Molecular characteristics of strains and clinical and demographic parameters of 11 patients from whom Mycobacterium bovis was isolated, southwest Ireland, 1998–2006*

Study no. Spoligotype SpolDB4† Mbovis. org‡ Other name§ VNTR pattern¶ Patient data
Sex/ age, y Sample site Sputum smear
HB002 ■■□■■□■□□□□□■■■□■■■■■■■■■■■■■■■■■■■■■■□□□□□ ST683 SB0140 A1 4-11-4-7-5-
1-5-8-3-2 F/81 Sputum +
HB008 ■■□■■□■□□□□□■■■□■■■■■■■■■■■■■■■■■■■■■■□□□□□ ST683 SB0140 A1 3-10-4-5-5-
1-5-8-3-4 F/86 Sputum
HB010 ■■□■■□■□□□□□■■■□■■■■■■■■■■■■■■■■■■■■■■□□□□□ ST683 SB0140 A1 4-3-4-7-5-
1-5-8-3-4 F/33 Sputum +
HB011 ■■□■■□■□□□□□■■■□■■■■■■■■■■■■■■■■□■■■■■□□□□□ ST680 SB0139 NA 4-3-4-7-3-
1-5-8-3-4 F/29 Sputum +
HB012 ■■□■■□■□□□□□■■■□■■■■■■■■■■■■■■■■□■■■■■□□□□□ ST680 SB0139 NA 4-10-4-7-4-
1-5-8-3-4 M/62 Sputum +
HB006 ■■□■■□■□□□□□■■■□■■■■■■■■■■■■■■■■■■□■■■□□□□□ ND SB1186 NA 4-11-4-5-5-
2-5-9-4-4 M/60 Urine NA
HB007 ■■□■■□■□□□□□■■■□■■■■■■■■■■■■■■■■■■□□■■□□□□□ ND SB1047 NA 4-10-3-7-7-
2-4-x-4-2 F/74 Neck abscess NA
HB003 ■■□■■□■□□□□□■■■□■■■■■■■■■■■■■■■■□□■■■■□□□□□ ST679 SB0142 D1 3-11-4-7-5-
1-6-8-3-4 M/37 Spinal disc aspirate NA
HB009 ■■□■■□■□□□□□■■■□■■■■■■■■■■■■■■■■■■□■□■□□□□□ ND SB1187 NA 4-11-4-7-5-
2-5-10-4-4 F/80 Sputum +
HB001 ■■□■■■■■□■□■■■■□■■■■■■■■■■■■■■■■■■■■■■□□□□□ ST691 SB130 A2 2-11-3-7-5-
2-3-8-2-3 M/16 Sputum +
HB005 ■■□■■■■■□■□■■■■□■■■□□■■■■■■■■■■■■■■■■■□□□□□ ND SB1185 NA 4-10-1-4-5-
2-6-11-7-4 M/68 Testis biopsy sample NA

*VNTR, variable number tandem repeats; +, smear positive by auramine and Ziehl-Neelsen stains; −, smear negative by auramine and Ziehl-Neelsen stains; NA, not applicable; ND, not described; x, not determined.
†Spoligotype designation as described in the SpolDB4 database (
‡Spoligotype assignment as described in the database (
§Spoligotype assignment (11).
¶VNTR profile based on a 10-loci scheme for M. bovis strains from Ireland in this order: QUB1895, QUB11a, QUB11b, ETR-A, ETR-B, MIRU 24, MIRU 26, QUB 3232, QUB 3336, and QUB26 (8).

Main Article

  1. de la Rua-Domenech  R. Human Mycobacterium bovis infection in the United Kingdom: incidence, risks, control measures and review of the zoonotic aspects of bovine tuberculosis. Tuberculosis (Edinb). 2006;86:77109. DOIPubMedGoogle Scholar
  2. Cadmus  S, Palmer  S, Okker  M, Dale  J, Gover  K, Smith  N, Molecular analysis of human and bovine tubercle bacilli from a local setting in Nigeria. J Clin Microbiol. 2006;44:2934. DOIPubMedGoogle Scholar
  3. Reviriego Gordejo  FJ, Vermeersch  JP. Towards eradication of bovine tuberculosis in the European Union. Vet Microbiol. 2006;112:1019. DOIPubMedGoogle Scholar
  4. More  SJ, Good  M. The tuberculosis eradication programme in Ireland: a review of scientific and policy advances since 1988. Vet Microbiol. 2006;112:23951. DOIPubMedGoogle Scholar
  5. Cotter  TP, Sheehan  S, Cryan  B, O’Shaughnessy  E, Cummins  H, Bredin  CP. Tuberculosis due to Mycobacterium bovis in humans in the south-west region of Ireland: is there a relationship with infection prevalence in cattle? Tuber Lung Dis. 1996;77:5458. DOIPubMedGoogle Scholar
  6. Evans  JT, Smith  EG, Banerjee  A, Smith  RM, Dale  J, Innes  JA, Cluster of human tuberculosis caused by Mycobacterium bovis: evidence for person-to-person transmission in the UK. Lancet. 2007;369:12706. DOIPubMedGoogle Scholar
  7. Frothingham  R, Meeker-O’Connell  WA. Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats. Microbiology. 1998;144:118996.PubMedGoogle Scholar
  8. Roring  S, Scott  AN, Glyn Hewinson  R, Neill  SD, Skuce  RA. Evaluation of variable number tandem repeat (VNTR) loci in molecular typing of Mycobacterium bovis isolates from Ireland. Vet Microbiol. 2004;101:6573. DOIPubMedGoogle Scholar
  9. Kamerbeek  J, Schouls  L, Kolk  A, van Agterveld  M, van Soolingen  D, Kuijper  S, Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997;35:90714.PubMedGoogle Scholar
  10. Brosch  R, Gordon  SV, Marmiesse  M, Brodin  P, Buchrieser  C, Eiglmeier  K, A new evolutionary scenario for the Mycobacterium tuberculosis complex. Proc Natl Acad Sci U S A. 2002;99:36849. DOIPubMedGoogle Scholar
  11. Costello  E, O’Grady  D, Flynn  O, O’Brien  R, Rogers  M, Quigley  F, Study of restriction fragment length polymorphism analysis and spoligotyping for epidemiological investigation of Mycobacterium bovis infection. J Clin Microbiol. 1999;37:321722.PubMedGoogle Scholar
  12. Smith  NH, Dale  J, Inwald  J, Palmer  S, Gordon  SV, Hewinson  RG, The population structure of Mycobacterium bovis in Great Britain: clonal expansion. Proc Natl Acad Sci U S A. 2003;100:152715. DOIPubMedGoogle Scholar
  13. Lari  N, Rindi  L, Bonanni  D, Tortoli  E, Garzelli  C. Molecular analysis of clinical isolates of Mycobacterium bovis recovered from humans in Italy. J Clin Microbiol. 2006;44:421821. DOIPubMedGoogle Scholar
  14. Mignard  S, Pichat  C, Carret  G. Mycobacterium bovis infection, Lyon, France. Emerg Infect Dis. 2006;12:14313.PubMedGoogle Scholar
  15. Supply  P, Allix  C, Lesjean  S, Cardoso-Oelemann  M, Rusch-Gerdes  S, Willery  E, Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis. J Clin Microbiol. 2006;44:4498510. DOIPubMedGoogle Scholar

Main Article

1Current affiliation: University College Dublin, Dublin, Ireland.

Page created: July 22, 2010
Page updated: July 22, 2010
Page reviewed: July 22, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.