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Volume 14, Number 4—April 2008
Dispatch

Dengue Virus Type 4, Manaus, Brazil

Regina Maria Pinto de Figueiredo*1Comments to Author , Felipe Gomes Naveca†1, Michele de Souza Bastos*1, Miriam do Nascimento Melo*, Suziane de Souza Viana‡, Maria Paula Gomes Mourão*, Cristóvão Alves Costa‡, and Izeni Pires Farias§

Author affiliations: *Fundação de Medicina Tropical do Amazonas, Manaus, Brazil; †Fundação Alfredo da Matta, Manaus, Brazil; ‡Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil; §Universidade Federal do Amazonas, Manaus, Brazil

Main Article

Table

Results of different methods used to confirm dengue virus type 4 (DENV-4) infection, Manaus, Amazonas, Brazil*

Isolate IgM antibody capture ELISA Virus culture†
M-PCR
S-PCR
BLAST
D1 D2 D3 D4 D1 D2 D3 D4 D1 D2 D3 D4
AM750 + + + + + + DENV-3/ DENV-4‡
AM1041 + + + + NS
AM1619 + + + + DENV-4

*IgM, immunoglobulin M; M-PCR, multiplex typing PCR described by Lanciotti et al. (10); S-PCR, single serotype-specific primer PCR; D, dengue serotype; BLAST, basic local alignment search tool; NS, not sequenced.
†Viral antigens detected by immunofluorescence with type-specific monoclonal antibodies.
‡Isolate AM750 is from a person with a co-infection; different clones were sequenced.

Main Article

References
  1. Gubler  DJ. Dengue and dengue haemorrhagic fever. Clin Microbiol Rev. 1998;11:48096.PubMedGoogle Scholar
  2. Gubler  DJ. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol. 2002;10:1003. DOIPubMedGoogle Scholar
  3. World Health Organization. Dengue and dengue haemorrhagic fever. 2002 [cited 2008 Jan 9]. Available from http://www.who.int/mediacentre/factsheets/fs117/en
  4. Ministério da Saúde. Informe epidemiológico do SUS. 2000. Brasília (Brazil): Ministério da Saúde Press; 2000.
  5. Figueiredo  RM, Thatcher  BD, Lima  ML, Almeida  TC, Alecrim  WD, Guerra  MV. Exanthematous diseases and the first epidemic of dengue to occur in Manaus, Amazonas, State, Brazil, during 1998–1999 [in Portuguese]. Rev Soc Bras Med Trop. 2004;37:4769. DOIPubMedGoogle Scholar
  6. Figueiredo  RM, Bastos  MS, Lima  ML, Almeida  TC, Alecrim  WD. Dinâmica da sorologia e isolamento viral na epidemia de dengue em Manaus (1998–2001). In: Abstracts of the 38th Congress of Brazilian Society of Tropical Medicine. Foz do Iguaçu, Paraná, Brazil; 2002 Feb 24–28. Brazilian Society of Tropical Medicine; 2002. p. 94.
  7. Figueiredo  RM, Silva  MN, Almeida  TC, Lopes  HC, Bastos  MS. Diagnóstico virológico de dengue em pacientes com quadro febril não diferenciado. In: Abstracts of the 39th Congress of Brazilian Society of Tropical Medicine. Belém, Pará, Brazil; 2003 Mar 11–15. Brazilian Society of Tropical Medicine; 2003. p. 485–6.
  8. Igarashi  A. Isolation of a Singh’s Aedes albopictus cell clone sensitive to dengue and chikungunya viruses. J Gen Virol. 1978;40:53144.PubMedGoogle Scholar
  9. Kuno  G, Gomez  I, Gubler  DJ. Detecting artificial antidengue IgM immune complexes using an enzyme-linked immunosorbent assay. Am J Trop Med Hyg. 1987;36:1539.PubMedGoogle Scholar
  10. Lanciotti  RS, Calisher  CH, Gubler  DJ, Chang  GJ, Vorndam  AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol. 1992;30:54551.PubMedGoogle Scholar
  11. Ocazionez  RE, Cortes  FM, Villar  LA, Gomez  SY. Temporal distribution of dengue virus serotypes in Colombian endemic area and dengue incidence. Reintroduction of dengue-3 associated to mild febrile illness and primary infection. Mem Inst Oswaldo Cruz. 2006;101:72531. DOIPubMedGoogle Scholar
  12. Lorono Pino  MA, Farfan Ale  JA, Rosado Paredes  EP, Kuno  G, Gubler  DJ. Epidemic dengue 4 in the Yucatan, Mexico, 1984. Rev Inst Med Trop Sao Paulo. 1993;35:44955. DOIPubMedGoogle Scholar
  13. Bravo  JR, Guzmãn  MG, Kourí  GP. Why dengue haemorrhagic fever in Cuba? I. Individual risk factors for dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS). Trans R Soc Trop Med Hyg. 1987;81:81620. DOIPubMedGoogle Scholar
  14. Osanai  CH. A epidemia de Dengue em Boa Vista, território Federal de Roraima, 1981−1982 [dissertation]. Rio de Janeiro, Brazil: Escola Nacional de Saúde Pública; 1984.
  15. Lanciotti  RS, Gubler  DJ, Trent  DW. Molecular evolution and phylogeny of dengue-4 viruses. J Gen Virol. 1997;78:227986.PubMedGoogle Scholar

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1These authors contributed equally to this work.

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