Volume 14, Number 6—June 2008
Research
Influenza A Virus (H3N8) in Dogs with Respiratory Disease, Florida
Sunchai Payungporn*, P. Cynda Crawford†, Theodore S. Kouo*, Li-mei Chen*, Justine Pompey*, William L. Castleman†, Edward J. Dubovi‡, Jacqueline M. Katz*, and Ruben O. Donis*
Table 1
Primers and probes for identification of canine influenza virus (H3N8) in tissues by quantitative real-time reverse transcription–PCR*
| Primer | Target | Sequence | Application |
|---|---|---|---|
| Ca-H3-F387 | H3 (nt 387–406) | 5′-tatgcatcgctccgatccat-3′ | Forward primer for H3 |
| Ca-H3-R487 | H3 (nt 487–467) | 5′-gctccacttcttccgttttga-3′ | Reverse primer for H3 |
| Ca-H3-P430 | H3 (nt 430–459) | FAM-aattcacagcagagggattcacatggacag-BHQ1 | TaqMan probe |
| FluA-M-F151 | M (nt 151–174) | 5′-catggartggctaaagacaagacc-3′ | Forward primer for M |
| FluA-M-R276 | M (nt 276–253) | 5′-agggcattttggacaaakcgtcta-3′ | Reverse primer for M |
| FluA-M-P218 | M (nt 218–235) | FAM-acgcTcaccgTgcccAgt-BHQ1 | TaqMan probe |
*Conserved regions of the matrix (M) and hemagglutinin 3 (H3) genes of canine influenza virus were selected. The underlined r represents a mixture of a and g nucleotides at this position in the oligonucleotide; the underlined k represents mixtures of g or t; uppercase letters in the sequence for the M probe represent locked nucleic acids.
Page created: July 09, 2010
Page updated: July 09, 2010
Page reviewed: July 09, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.