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Volume 14, Number 6—June 2008
Research

Influenza A Virus (H3N8) in Dogs with Respiratory Disease, Florida

Sunchai Payungporn*, P. Cynda Crawford†, Theodore S. Kouo*, Li-mei Chen*, Justine Pompey*, William L. Castleman†, Edward J. Dubovi‡, Jacqueline M. Katz*, and Ruben O. Donis*Comments to Author 
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †University of Florida, Gainesville, Florida, USA; ‡Cornell University, Ithaca, New York, USA;

Main Article

Table 2

Quantitative real-time reverse transcription–PCR and virus isolation from tissue specimens*

Dog Location Duration of
clinical disease, d Real-time reverse transcription–PCR
18S rRNA (Ct) M (Ct) M:rRNA H3 (Ct) H3:rRNA Virus isolation
1079 Shelter 2 26.18 29.81 1.14 28.84 1.10 NVD
1078 Shelter 3 26.82 30.37 1.13 29.71 1.11 MDCK cells
3rd passage
1080 Shelter 6 24.17 38.87 1.61 38.23 1.58 NVD
319 Shelter 6 24.94 33.87 1.36 32.23 1.29 NVD
318 Shelter 9 23.54 33.89 1.44 32.97 1.40 NVD
320 Shelter 10 23.91 39.44 1.65 37.09 1.55 NVD
374 Veterinary clinic 3 22.89 24.05 1.05 22.65 0.99 Egg
2nd passage
A/canine/Florida/242/2003 29.37 28.15 0.96 27.36 0.93 NA
Normal dog lung 20.78 >40 >1.92 >40 >1.92 NA

*Ct, cycle threshold; NVD, no virus detected; NA, not applicable; M:rRNA and H3:rRNA ratios were calculated by dividing the matrix (M) or hemagglutinin 3 (H3) Ct by the 18S rRNA Ct.

Main Article

Page created: July 09, 2010
Page updated: July 09, 2010
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