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Volume 14, Number 7—July 2008

Experimental Infection of Cattle with Highly Pathogenic Avian Influenza Virus (H5N1)

Donata Kalthoff*, Bernd Hoffmann*, Timm Harder*, Markus Durban*, and Martin Beer*Comments to Author 
Author affiliations: *Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany;

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Table 1

Detection of highly pathogenic avian influenza virus (H5N1) in nasal swabs from calves, Germany, 2007*

Calf Day postinoculation
PCR† PCR Egg Cell PCR Egg Cell PCR
A1 >40 20–30 30–40 >40
A2 >40 30–40 + + 30–40 + >40
A3 >40 20–30 + + >40 ND ND >40



K1 >40 >40 ND ND >40 ND ND >40
K2 >40 >40 ND ND >40 ND ND >40

*Inoculated calves received highly pathogenic influenza virus (H5N1) strain A/cat/Germany/R606/2006 (8,9); contact calves were not inoculated but were housed with the inoculated calves. Egg, embryonated chicken eggs; cell, MDCK cell culture; +, virus detected; –, virus not detected; ND, not done.
†H5-specific real-time reverse transcription­–PCR; results are shown as range of detected threshold cycle values.

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