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Volume 14, Number 9—September 2008

Circulation of 3 Lineages of a Novel Saffold Cardiovirus in Humans

Ana Maria Bispo de Filippis, Luciano Kleber de Souza Luna, Andreas Stöcker, Patrícia Silva Almeida, Tereza Cristina Medrado Ribeiro, Nadine Petersen, Petra Herzog, Célia Pedroso, Hans Iko Huppertz, Hugo da Costa Ribeiro, Sigrid Baumgarte, and Sung Sup ParkComments to Author 
Author affiliations: Federal University of Bahia, Salvador, Brazil (J.F. Drexler, A. Stöcker, P. Silva Almeida, T.C. Medrado Ribeiro, C. Pedroso, H. da Costa Ribeiro Jr.); Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (J.F. Drexler, L.K. de Souza Luna, N. Petersen, P. Herzog); Professor Hess Paediatric Hospital, Bremen, Germany (H.I. Huppertz); Institute of Hygiene and the Environment, Hamburg (S. Baumgarte); University of Bonn Medical Centre, Bonn, Germany (C. Drosten);

Main Article

Table 4

VP1 amino acid identity between sequences*†

Taxon/species Strain [1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13]
Saf-1 [1] Saffold original
Saf-2 [2] SafV Canada 77.3
[3] D/VI2229/2004 77.3 98.5
[4] BR/118/2006 77.7 97.8 98.2
Saf-3 [5] D/VI2273/2004 67.9 73.4 73.1 73.1
[6] D/VI2223/2004 67.9 73.4 73.1 73.1 100.0
Theilovirus [7] TMEV GDVII 57.9 58.3 58.3 58.3 56.7 56.7
[8] TMEV DA 59.1 61.0 60.6 60.6 57.8 57.8 90.9
[9] TMEV BeAn 57.2 58.7 58.7 59.0 56.7 56.7 92.8 93.1
[10] Rat Theiler-like 59.0 57.9 57.9 57.9 55.9 55.9 72.8 73.7 74.3
[11] Vilyuisk 60.3 56.6 56.6 57.0 57.4 57.4 71.7 71.5 71.7 69.6
Encephalomyocarditis virus [12] EMCV 48.4 49.3 49.3 49.3 46.7 46.7 49.4 50.2 49.4 49.1 49.6
[13] Mengo 49.1 50.7 50.7 51.1 47.4 47.4 50.9 51.3 50.9 49.8 50.4 95.7

*VP1, viral protein 1; SafV, Saffold-like virus; TMEV, Theiler’s murine encephalomyelitis virus; EMCV, murine encephalomyocarditis virus.
†The percentage of amino acid identity per site from analysis between sequences is shown. All results are based on the pairwise analysis of 13 sequences (pairwise deletion option). Analyses were conducted by using MEGA version 4 (24). The final dataset contained a total of 287 positions. GenBank accession numbers are given in the Materials and Methods section.

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