Volume 15, Number 12—December 2009
Research
Novel Calicivirus Identified in Rabbits, Michigan, USA
Ingrid L. Bergin
, Annabel G. Wise, Steven R. Bolin, Thomas P. Mullaney, Matti Kiupel, and Roger K. Maes
, Annabel G. Wise, Steven R. Bolin, Thomas P. Mullaney, Matti Kiupel, and Roger K. Maes
Table
Primers used in genomic sequencing of Michigan rabbit calicivirus*
| Genomic region(s) spanned | Forward and reverse primer pairs† | Nucleotide positions in RHDV‡ |
|---|---|---|
| Polymerase-capsid (VP60) | 5′-ATGCCATGACTCCGATGATGGT-3′ (+) R | 4835–4856 |
| 5′-CTTGTTGGTCCACCTGTTG-3′ (−) M |
5482–5500 |
|
| Polymerase | 5′- GCGACTTCTTGTGCTTGGACTAC-3′ (+) R | 4493–4515 |
| 5′- GCACCACTCCAACTGTCTGAGAA-3′ (−) M |
5027–5049 |
|
| Polymerase | 5′-GTGACCCAGACAGTGACAAGT-3′ (+) R | 3929–3949 |
| 5′-GGCCTATTTCTGCACATGCTT-3′ (−) M |
4754–4774 |
|
| Protease-polymerase | 5′-GCGGTGACCARGGTGTTGATG-3′ (+) R | 2819–2839 |
| 5′-GCCGCAGCACGCTCTATGAAT-3′ (−) M |
4020–4040 |
|
| VPg-protease | 5′-AACAAAGCCGTTGAAAGTTGG-3′ (+) R | 1927–1947 |
| 5′-TGGCAGCTCTGTTCTTCATTT-3′ (−) M |
3119–3139 |
|
| Helicase | 5′-GAGGTTGTTTGACACGTTTGA-3′ (+) R | 1089–1109 |
| 5′-TGTCATATTCACACAGCCCAG -3′ (−) M |
2398–2418 |
|
| Capsid (VP60)–ORF 2 | 5′-GTTACGACAGTGCAGGCCTATG-3′ (+) M | 6412–6433 |
| 5′-CTCGCCAGTGGTGTTATAAATC -3′ (−) R | 7345–7366 |
*RHDV, rabbit hemorrhagic disease virus; MRCV, Michigan rabbit calicivirus; VP, viral protein; ORF, open reading frame.
†RHDV-specific primer denoted by R; MRCV-specific primer denoted by M.
‡Positions spanned by primer set in RHDV genome (Italy BS89 strain, GenBank accession no. X87607).
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