Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 15, Number 12—December 2009

CTX-M β-Lactamase Production and Virulence of Escherichia coli K1

Damien Dubois, Nemani V. Prasadarao, Rahul Mittal, Laurent Bret, Marie Roujou-Gris, and Richard BonnetComments to Author 
Author affiliations: University of Auvergne, Clermont-Ferrand, France (D. Dubois, R. Bonnet); Teaching Hospital, Clermont-Ferrand (D. Dubois, R. Bonnet); Childrens Hospital, Los Angeles, California, USA (N.V. Prasadarao, R. Mittal); University of Southern California, Los Angeles (N.V. Prasadarao); La Source Hospital, Orléans, France (L. Bret, M. Roujou-Gris)

Main Article

Table 1

Virulence factors of Orl-1 Escherichia coli K1 strain, France

Integrative elements* (1) Virulence genes Present
PAI III536–like iroN Yes

ibeA (gimA4), ptnC (gimA1)
PAI IIJ96–like hra Yes

hlyC, cnf1
PAI ICFT073–like hlyC No

aer (iucC)
fyuA, irp-2
pks island†
clbA, clbK-J, clbP, clbQ
Others chuA Yes
ompA Yes
hek Yes
iss Yes
malX Yes
cdtB-I to -V No

*PAI, pathogenicity island; HPI, high-pathogenicity island.
†Positive cytopathogen effect with transient infection of HeLa cells.

Main Article

  1. Bonacorsi  S, Clermont  O, Houdouin  V, Cordevant  C, Brahimi  N, Marecat  A, Molecular analysis and experimental virulence of French and North American Escherichia coli neonatal meningitis isolates: identification of a new virulent clone. J Infect Dis. 2003;187:1895906. DOIPubMedGoogle Scholar
  2. Pitout  JD, Laupland  KB. Extended-spectrum beta–lactamase-producing Enterobacteriaceae: an emerging public-health concern. Lancet Infect Dis. 2008;8:15966. DOIPubMedGoogle Scholar
  3. Lavigne  JP, Blanc-Potard  AB, Bourg  G, Moreau  J, Chanal  C, Bouziges  N, Virulence genotype and nematode-killing properties of extra-intestinal Escherichia coli producing CTX-M beta-lactamases. Clin Microbiol Infect. 2006;12:1199206. DOIPubMedGoogle Scholar
  4. Pitout  JD, Laupland  KB, Church  DL, Menard  ML, Johnson  JR. Virulence factors of Escherichia coli isolates that produce CTX-M-type extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 2005;49:466770. DOIPubMedGoogle Scholar
  5. Branger  C, Zamfir  O, Geoffroy  S, Laurans  G, Arlet  G, Thien  HV, Genetic background of Escherichia coli and extended-spectrum beta-lactamase type. Emerg Infect Dis. 2005;11:5461.PubMedGoogle Scholar
  6. Andersson  DI. The biological cost of mutational antibiotic resistance: any practical conclusions? Curr Opin Microbiol. 2006;9:4615. DOIPubMedGoogle Scholar
  7. Houdouin  V, Bonacorsi  S, Bidet  P, Bingen  E. Antimicrobial susceptibility of 136 Escherichia coli isolates from cases of neonatal meningitis and relationship with virulence. Clin Microbiol Infect. 2007;13:120710. DOIPubMedGoogle Scholar
  8. Galiza  EP, Heath  PT. Improving the outcome of neonatal meningitis. Curr Opin Infect Dis. 2009;22:22934. DOIPubMedGoogle Scholar
  9. Jonas  D, Spitzmuller  B, Weist  K, Ruden  H, Daschner  FD. Comparison of PCR-based methods for typing Escherichia coli. Clin Microbiol Infect. 2003;9:82331. DOIPubMedGoogle Scholar
  10. Prasadarao  NV, Wass  CA, Weiser  JN, Stins  MF, Huang  SH, Kim  KS. Outer membrane protein A of Escherichia coli contributes to invasion of brain microvascular endothelial cells. Infect Immun. 1996;64:14653.PubMedGoogle Scholar
  11. Peigne  C, Bidet  P, Mahjoub-Messai  F, Plainvert  C, Barbe  V, Medigue  C, The plasmid of neonatal meningitis Escherichia coli strain S88 (O45:K1:H7) is closely related to avian pathogenic E. coli plasmids and is associated with high level bacteremia in a neonatal rat meningitis model. Infect Immun. 2009;77:227284. DOIPubMedGoogle Scholar
  12. Bizzarro  MJ, Dembry  LM, Baltimore  RS, Gallagher  PG. Changing patterns in neonatal Escherichia coli sepsis and ampicillin resistance in the era of intrapartum antibiotic prophylaxis. Pediatrics. 2008;121:68996. DOIPubMedGoogle Scholar
  13. Boyer-Mariotte  S, Duboc  P, Bonacorsi  S, Lemeland  JF, Bingen  E, Pinquier  D. CTX-M-15–producing Escherichia coli in fatal neonatal meningitis: failure of empirical chemotherapy. J Antimicrob Chemother. 2008;62:14724. DOIPubMedGoogle Scholar
  14. Ramdani-Bouguessa  N, Mendonca  N, Leitao  J, Ferreira  E, Tazir  M, Canica  M. CTX-M-3 and CTX-M-15 extended-spectrum beta-lactamases in isolates of Escherichia coli from a hospital in Algiers, Algeria. J Clin Microbiol. 2006;44:45846. DOIPubMedGoogle Scholar
  15. Sehgal  R, Gaind  R, Chellani  H, Agarwal  P. Extended-spectrum beta lactamase–producing gram-negative bacteria: clinical profile and outcome in a neonatal intensive care unit. Ann Trop Paediatr. 2007;27:4554. DOIPubMedGoogle Scholar

Main Article

Page created: December 09, 2010
Page updated: December 09, 2010
Page reviewed: December 09, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.