Figure 3. A) Difference in pigmentation of the low hemolytic (LH)/high encapsulation (HC) and NEM316 wild-type (WT) strains and their corresponding transformants expressing the covR/S locus of the high hemolytic (HH)/low encapsulation (LC) variant after overnight culture. B) CAMP testing with strains displayed according to panel A. The LH/HC variant and the NEM316 strain display a stronger reaction (arrows) than their corresponding transformants. C) Semiquantitative analysis of mRNA expression of CAMP factor (cftb), β-h/c (cylK and cylE) capsule (cpsG), and gyrA (housekeeping gene) using reverse transcription–PCR. Lane 1, LH/HC; lane 2, HH/LC; lane 3, LH/HC + pcovR HH; lane 4, NEM316 WT; lane 5, NEM316 + pcovR HH.