Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 15, Number 3—March 2009

Coordinated Implementation of Chikungunya Virus Reverse Transcription–PCR

Marcus Panning, Remi N. Charrel, Oliver D. Mantke, Olfert Landt, Matthias Niedrig, and Sung Sup ParkComments to Author 
Author affiliations: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (M. Panning); Université de la Méditerranée, Marseille, France (R.N. Charrel); Robert Koch Institute, Berlin, Germany (O.D. Mantke, M. Niedrig); TIB MOLBIOL, Berlin (O. Landt); University of Bonn Medical Centre, Bonn, Germany (C. Drosten)

Main Article

Table 2

Possible technical factors influencing performance of laboratories in detection of CHIKV*

Factor No. laboratories p value for positive influence on sensitivity
QIAGEN† viral RNA extraction kit 23 0.2
Any automated RNA extraction procedure 8 0.08
Preformulated CHIKV real-time RT-PCR protocol 13 0.03
Any real-time CHIKV RT-PCR 27 0.3
Any nested CHIKV RT-PCR 6 0.37

*CHIKV, chikungunya virus; RT-PCR, reverse transcription–PCR.
†Hilden, Germany.

Main Article

Page created: December 07, 2010
Page updated: December 07, 2010
Page reviewed: December 07, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.