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Volume 15, Number 5—May 2009
Research

CTX-M β-Lactamases in Escherichia coli from Community-acquired Urinary Tract Infections, Cambodia

Etienne RuppéComments to Author , Sopheak Hem, Sovannarith Lath, Valérie Gautier, Frédéric Ariey, Jean-Louis Sarthou, Didier Monchy, and Guillaume Arlet
Author affiliations: Institut Pasteur du Cambodge, Phnom Penh, Cambodia (E. Ruppé, S. Hem, S. Lath, F. Ariey, J.-L. Sarthou, D. Monchy); Université Pierre et Marie Curie-Paris Faculté de Médecine, Paris, France (V. Gautier, G. Arlet); Assistance Publique-Hôpitaux de Paris–Hôpital Tenon, Paris (V. Gautier, G. Arlet)

Main Article

Table 3

Mutations detected in the ampC promoter region of CTX-M β-lactam–resistant Escherichia coli*

Isolate CEF MIC, mg/L CTX-M Phylogenetic group Mutation at position†
−42 −32 −28 −18 −1 +22 +26 +27 +32 +58 +70 +81
E. coli K12 (U00096) C T G G C C T A G C C G
CEC1 16 Yes D T G T A T
CEC3 32 No D T G T A T
CEC4 32 No D A T T
CEC6 32 Yes D T G T A T
CEC8 32 Yes D T G T A T
CEC9 32 Yes D T G T A T
CEC10 32 Yes D T G T A T
CEC13 32 Yes D T G T A T
CEC14 16 Yes D T
CEC15 16 Yes D T
CEC19 32 No D T G T A T
CEC21 16 Yes D A T A
CEC22 16 Yes D T
CEC31 16 Yes D A T A
CEC57 32 No D A T G T A T
CEC88 32 No B2 A A
CEC89 32 Yes D T G T A T
CEC92 >128 Yes D T A T T
CEC93 >128 No D T G T A T

*CEF, cefoxitin; CEC, Cambodian E. coli.
†According to numbering of Jaurin and Grundström (30).

Main Article

References
  1. Ambler  RP. The structure of beta-lactamases. Philos Trans R Soc Lond B Biol Sci. 1980;289:32131. DOIPubMedGoogle Scholar
  2. Hall  BG, Barlow  M. Revised Ambler classification of β-lactamases. J Antimicrob Chemother. 2005;55:10501. DOIPubMedGoogle Scholar
  3. Bush  K, Jacoby  GA, Medeiros  AA. A functional classification scheme for beta-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother. 1995;39:121133.PubMedGoogle Scholar
  4. Humeniuk  C, Arlet  G, Gautier  V, Grimont  P, Labia  R, Philippon  A. Beta-lactamases of Kluyvera ascorbata, probable progenitors of some plasmid-encoded CTX-M types. Antimicrob Agents Chemother. 2002;46:30459. DOIPubMedGoogle Scholar
  5. Poirel  L, Kampfer  P, Nordmann  P. Chromosome-encoded Ambler class A beta-lactamase of Kluyvera georgiana, a probable progenitor of a subgroup of CTX-M extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 2002;46:403840. DOIPubMedGoogle Scholar
  6. Olson  AB, Silverman  M, Boyd  DA, McGeer  A, Willey  BM, Pong-Porter  V, Identification of a progenitor of the CTX-M-9 group of extended-spectrum beta-lactamases from Kluyvera georgiana isolated in Guyana. Antimicrob Agents Chemother. 2005;49:21125. DOIPubMedGoogle Scholar
  7. Bonnet  R, Recule  C, Baraduc  R, Chanal  C, Sirot  D, De Champs  C, Effect of D240G substitution in a novel ESBL CTX-M-27. J Antimicrob Chemother. 2003;52:2935. DOIPubMedGoogle Scholar
  8. Karim  A, Poirel  L, Nagarajan  S, Nordmann  P. Plasmid-mediated extended-spectrum beta-lactamase (CTX-M-3 like) from India and gene association with insertion sequence ISEcp1. FEMS Microbiol Lett. 2001;201:23741.PubMedGoogle Scholar
  9. Bradford  PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev. 2001;14:93351. DOIPubMedGoogle Scholar
  10. Canton  R, Coque  TM. The CTX-M beta-lactamase pandemic. Curr Opin Microbiol. 2006;9:46675. DOIPubMedGoogle Scholar
  11. Arpin  C, Coulange  L, Dubois  V, Andre  C, Fischer  I, Fourmaux  S, Extended-spectrum-beta-lactamase-producing Enterobacteriaceae strains in various types of private health care centers. Antimicrob Agents Chemother. 2007;51:34404. DOIPubMedGoogle Scholar
  12. Woodford  N, Ward  ME, Kaufmann  ME, Turton  J, Fagan  EJ, James  D, Community and hospital spread of Escherichia coli producing CTX-M extended-spectrum beta-lactamases in the UK. J Antimicrob Chemother. 2004;54:73543. DOIPubMedGoogle Scholar
  13. Ho  PL, Poon  WW, Loke  SL, Leung  MS, Chow  KH, Wong  RC, Community emergence of CTX-M type extended-spectrum beta-lactamases among urinary Escherichia coli from women. J Antimicrob Chemother. 2007;60:1404. DOIPubMedGoogle Scholar
  14. Lartigue  MF, Zinsius  C, Wenger  A, Bille  J, Poirel  L, Nordmann  P. Extended-spectrum beta-lactamases of the CTX-M type now in Switzerland. Antimicrob Agents Chemother. 2007;51:285560. DOIPubMedGoogle Scholar
  15. Minarini  LA, Gales  AC, Palazzo  IC, Darini  AL. Prevalence of community-occurring extended spectrum beta-lactamase–producing Enterobacteriaceae in Brazil. Curr Microbiol. 2007;54:33541. DOIPubMedGoogle Scholar
  16. Mendonca  N, Leitao  J, Manageiro  V, Ferreira  E, Canica  M. Spread of extended-spectrum beta-lactamase CTX-M–producing Escherichia coli clinical isolates in community and nosocomial environments in Portugal. Antimicrob Agents Chemother. 2007;51:194655. DOIPubMedGoogle Scholar
  17. Calbo  E, Romani  V, Xercavins  M, Gomez  L, Vidal  CG, Quintana  S, Risk factors for community-onset urinary tract infections due to Escherichia coli harbouring extended-spectrum beta-lactamases. J Antimicrob Chemother. 2006;57:7803. DOIPubMedGoogle Scholar
  18. Rodriguez-Bano  J, Navarro  MD, Romero  L, Martinez-Martinez  L, Muniain  MA, Perea  EJ, Epidemiology and clinical features of infections caused by extended-spectrum beta-lactamase–producing Escherichia coli in nonhospitalized patients. J Clin Microbiol. 2004;42:108994. DOIPubMedGoogle Scholar
  19. Colodner  R, Rock  W, Chazan  B, Keller  N, Guy  N, Sakran  W, Risk factors for the development of extended-spectrum beta-lactamase–producing bacteria in nonhospitalized patients. Eur J Clin Microbiol Infect Dis. 2004;23:1637. DOIPubMedGoogle Scholar
  20. Jitsurong  S, Yodsawat  J. Prevalence of extended-spectrum beta-lactamases (ESBLs) produced in blood isolates of gram-negative bacteria in a teaching hospital in southern Thailand. Southeast Asian J Trop Med Public Health. 2006;37:1315.PubMedGoogle Scholar
  21. Kusum  M, Wongwanich  S, Dhiraputra  C, Pongpech  P, Naenna  P. Occurrence of extended-spectrum beta-lactamase in clinical isolates of Klebsiella pneumoniae in a university hospital, Thailand. J Med Assoc Thai. 2004;87:102933.PubMedGoogle Scholar
  22. Chanawong  A, M’Zali  FH, Heritage  J, Lulitanond  A, Hawkey  PM. SHV-12, SHV-5, SHV-2a and VEB-1 extended-spectrum beta-lactamases in Gram-negative bacteria isolated in a university hospital in Thailand. J Antimicrob Chemother. 2001;48:83952. DOIPubMedGoogle Scholar
  23. Saladin  M, Cao  VT, Lambert  T, Donay  JL, Herrmann  JL, Ould-Hocine  Z, Diversity of CTX-M beta-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals. FEMS Microbiol Lett. 2002;209:1618.PubMedGoogle Scholar
  24. Arlet  G, Rouveau  M, Philippon  A. Substitution of alanine for aspartate at position 179 in the SHV-6 extended-spectrum beta-lactamase. FEMS Microbiol Lett. 1997;152:1637.PubMedGoogle Scholar
  25. Caroff  N, Espaze  E, Berard  I, Richet  H, Reynaud  A. Mutations in the ampC promoter of Escherichia coli isolates resistant to oxyiminocephalosporins without extended spectrum beta-lactamase production. FEMS Microbiol Lett. 1999;173:45965.PubMedGoogle Scholar
  26. Poirel  L, Naas  T, Guibert  M, Chaibi  EB, Labia  R, Nordmann  P. Molecular and biochemical characterization of VEB-1, a novel class A extended-spectrum beta-lactamase encoded by an Escherichia coli integron gene. Antimicrob Agents Chemother. 1999;43:57381.PubMedGoogle Scholar
  27. Lavollay  M, Mamlouk  K, Frank  T, Akpabie  A, Burghoffer  B, Ben Redjeb  S, Clonal dissemination of a CTX-M-15 beta-lactamase–producing Escherichia coli strain in the Paris area, Tunis, and Bangui. Antimicrob Agents Chemother. 2006;50:24338. DOIPubMedGoogle Scholar
  28. Eckert  C, Gautier  V, Saladin-Allard  M, Hidri  N, Verdet  C, Ould-Hocine  Z, Dissemination of CTX-M-type beta-lactamases among clinical isolates of Enterobacteriaceae in Paris, France. Antimicrob Agents Chemother. 2004;48:124955. DOIPubMedGoogle Scholar
  29. Clermont  O, Bonacorsi  S, Bingen  E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol. 2000;66:45558. DOIPubMedGoogle Scholar
  30. Jaurin  B, Grundström  T. ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type. Proc Natl Acad Sci U S A. 1981;78:4897901. DOIPubMedGoogle Scholar
  31. Caroff  N, Espaze  E, Gautreau  D, Richet  H, Reynaud  A. Analysis of the effects of –42 and –32 ampC promoter mutations in clinical isolates of Escherichia coli hyperproducing ampC. J Antimicrob Chemother. 2000;45:7838. DOIPubMedGoogle Scholar
  32. Picard  B, Garcia  JS, Gouriou  S, Duriez  P, Brahimi  N, Bingen  E, The link between phylogeny and virulence in Escherichia coli extraintestinal infection. Infect Immun. 1999;67:54653.PubMedGoogle Scholar
  33. Pitout  JD, Hanson  ND, Church  DL, Laupland  KB. Population-based laboratory surveillance for Escherichia coli–producing extended-spectrum beta-lactamases: importance of community isolates with blaCTX-M genes. Clin Infect Dis. 2004;38:173641. DOIPubMedGoogle Scholar
  34. Arpin  C, Dubois  V, Coulange  L, Andre  C, Fischer  I, Noury  P, Extended-spectrum beta-lactamase–producing Enterobacteriaceae in community and private health care centers. Antimicrob Agents Chemother. 2003;47:350614. DOIPubMedGoogle Scholar
  35. Grenet  K, Guillemot  D, Jarlier  V, Moreau  B, Dubourdieu  S, Ruimy  R, Antibacterial resistance, Wayampis Amerindians, French Guyana. Emerg Infect Dis. 2004;10:11503.PubMedGoogle Scholar
  36. Pitout  JD, Church  DL, Gregson  DB, Chow  BL, McCracken  M, Mulvey  MR, Molecular epidemiology of CTX-M–producing Escherichia coli in the Calgary Health Region: emergence of CTX-M-15–producing isolates. Antimicrob Agents Chemother. 2007;51:12816. DOIPubMedGoogle Scholar
  37. Mugnaioli  C, Luzzaro  F, De Luca  F, Brigante  G, Perilli  M, Amicosante  G, CTX-M–type extended-spectrum beta-lactamases in Italy: molecular epidemiology of an emerging countrywide problem. Antimicrob Agents Chemother. 2006;50:27006. DOIPubMedGoogle Scholar
  38. Branger  C, Zamfir  O, Geoffroy  S, Laurans  G, Arlet  G, Thien  HV, Genetic background of Escherichia coli and extended-spectrum beta-lactamase type. Emerg Infect Dis. 2005;11:5461.PubMedGoogle Scholar

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Page created: December 16, 2010
Page updated: December 16, 2010
Page reviewed: December 16, 2010
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