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Volume 15, Number 9—September 2009
Dispatch

Genetic Differences between Avian and Human Isolates of Candida dubliniensis

Brenda A. McManus, Derek J. Sullivan, Gary P. Moran, Christophe d’Enfert, Marie-Elisabeth Bougnoux, Miles A. Nunn, and David C. ColemanComments to Author 
Author affiliations: Dublin Dental School and Hospital, Dublin, Ireland (B.A. McManus, D.J. Sullivan, G.P. Moran, D.C. Coleman); Trinity College Dublin, Dublin (B.A. McManus, D.J. Sullivan, G.P. Moran, D.C. Coleman); Institut Pasteur, Paris, France (C. d’Enfert, M.-E. Bougnoux); National Environmental Research Council Centre for Ecology and Hydrology, Oxford, UK (M.A. Nunn)

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Figure

Neighbor-joining trees based on the polymorphic sites in Candida dubliniensis multilocus sequence typing (MLST) sequences. Bootstrap values >60% are indicated at cluster nodes. Avian-associated isolates are indicated in red. Numbers of polymorphic sites in isolates are indicated by scale bars. A) Isolates of MLST clade C1 defined by McManus et al. (7) showing location of avian-associated isolates in relation to human isolates in the same clade; human isolates were originally obtained in many

Figure. Neighbor-joining trees based on the polymorphic sites in Candida dubliniensis multilocus sequence typing (MLST) sequences. Bootstrap values >60% are indicated at cluster nodes. Avian-associated isolates are indicated in red. Numbers of polymorphic sites in isolates are indicated by scale bars. A) Isolates of MLST clade C1 defined by McManus et al. (7) showing location of avian-associated isolates in relation to human isolates in the same clade; human isolates were originally obtained in many countries. B) Neighbor-joining tree based on polymorphic sites in MLST sequences for each of 13 internal transcribed spacer genotype 1 C. dubliniensis isolates, 7 of which were obtained from humans in Ireland and 6 from seabird excrement in Ireland. Isolates that had identical diploid sequence types (DSTs) were not included in the tree so that only 1 of each DST is included. Tree displays the robustness of the avian-associated subgroup of isolates within a population of similar human-associated isolates from the same region. The rate of heterozygosity among human and avian-associated clade C1 isolates was 1.6 and 1 heterozygous site per DST, respectively, from 36 polymorphic sites, which indicated that avian-associated isolates were more clonal.

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References
  1. Buck  JD. Isolation of Candida albicans and halophilic Vibrio spp. from aquatic birds in Connecticut and Florida. Appl Environ Microbiol. 1990;56:8268.PubMedGoogle Scholar
  2. Edelmann  A, Kruger  M, Schmid  J. Genetic relationship between human and animal isolates of Candida albicans. J Clin Microbiol. 2005;43:61646. DOIPubMedGoogle Scholar
  3. Wrobel  L, Whittington  JK, Pujol  C, Oh  SH, Ruiz  MO, Pfaller  MA, Molecular phylogenetic analysis of a geographically and temporally matched collection of Candida albicans isolates from humans and nonmigratory wildlife in central Illinois. Eukaryot Cell. 2008;7:147586. DOIPubMedGoogle Scholar
  4. Jacobsen  MD, Bougnoux  ME, d’Enfert  C, Odds  FC. Multilocus sequence typing of Candida albicans isolates from animals. Res Microbiol. 2008;159:43640. DOIPubMedGoogle Scholar
  5. Nunn  MA, Schaefer  SM, Petrou  MA, Brown  JR. Environmental source of Candida dubliniensis. Emerg Infect Dis. 2007;13:74750.PubMedGoogle Scholar
  6. Odds  FC, Jacobsen  MD. Multilocus sequence typing of pathogenic Candida species. Eukaryot Cell. 2008;7:107584. DOIPubMedGoogle Scholar
  7. McManus  BA, Coleman  DC, Moran  G, Pinjon  E, Diogo  D, Bougnoux  ME, Multilocus sequence typing reveals that the population structure of Candida dubliniensis is significantly less divergent than that of Candida albicans. J Clin Microbiol. 2008;46:65264. DOIPubMedGoogle Scholar
  8. Moran  G, Sullivan  D, Morschhauser  J, Coleman  D. The Candida dubliniensis CdCDR1 gene is not essential for fluconazole resistance. Antimicrob Agents Chemother. 2002;46:282941. DOIPubMedGoogle Scholar
  9. Coleman  D, Sullivan  D, Harrington  B, Haynes  K, Henman  M, Shanley  D, Molecular and phenotypic analysis of Candida dubliniensis: a recently identified species linked with oral candidosis in HIV-infected and AIDS patients. Oral Dis. 1997;3(Suppl 1):S96101.PubMedGoogle Scholar
  10. Al Mosaid  A, Sullivan  DJ, Coleman  DC. Differentiation of Candida dubliniensis from Candida albicans on Pal’s agar. J Clin Microbiol. 2003;41:47879. DOIPubMedGoogle Scholar
  11. Pincus  DH, Coleman  DC, Pruitt  WR, Padhye  AA, Salkin  IF, Geimer  M, Rapid identification of Candida dubliniensis with commercial yeast identification systems. J Clin Microbiol. 1999;37:35339.PubMedGoogle Scholar
  12. Donnelly  SM, Sullivan  DJ, Shanley  DB, Coleman  DC. Phylogenetic analysis and rapid identification of Candida dubliniensis based on analysis of ACT1 intron and exon sequences. Microbiology. 1999;145:187182. DOIPubMedGoogle Scholar
  13. Gee  SF, Joly  S, Soll  DR, Meis  JF, Verweij  PE, Polacheck  I, Identification of four distinct genotypes of Candida dubliniensis and detection of microevolution in vitro and in vivo. J Clin Microbiol. 2002;40:55674. DOIPubMedGoogle Scholar
  14. Kumar  S, Tamura  K, Nei  M. MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform. 2004;5:15063. DOIPubMedGoogle Scholar
  15. Pujol  C, Daniels  KJ, Lockhart  SR, Srikantha  T, Radke  JB, Geiger  J, The closely related species Candida albicans and Candida dubliniensis can mate. Eukaryot Cell. 2004;3:101527. DOIPubMedGoogle Scholar

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