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Volume 16, Number 12—December 2010
Letter

Molecular Detection of Bartonella alsatica in Rabbit Fleas, France

Tahar Kernif, Philippe Parola, Jean-Claude Ricci, Didier Raoult, and Jean-Marc RolainComments to Author 
Author affiliations: Author affiliations: Université de la Méditerranée, Marseille, France (T. Kernif, P. Parola, D. Raoult, J.-M. Rolain); Institut Méditerranéen du Patrimoine Cynégétique et Faunistique, Vergèze, France (J.-C. Ricci)

Main Article

Table

Oligonucleotide primers and TaqMan* fluorescent probe sequences of hsp60 and gyrB genes used for reverse transcription PCRs of Bartonella alsatica†

Gene
 Oligonucleotide
 Sequence (5′ → 3′)
 Length, bp
 Amplicon size, bp
hsp60 B_alsa_hsp60_F TGCTAACGCTATGGAAAAAGTTG 23 108
B_alsa_hsp60_R CCACGATCAAACTGCATTCC 20
B_alsa_hsp60_P
 6FAM-TGTCGAAGAAGCAAAAACGGCTGAAACC-TAMRA
 28
gyrB B_alsa_gyrB_F CGAAGCAAAACTTCTTATTAGTAAGGT 27 126
B_alsa_gyrB_R GCAAGCTTTCCTGGCAGAG 19
B_alsa_gyrB_P 6FAM-ATAGAGGCTGCTGCGGCGCG-TAMRA 20

*Applied Biosystems, Courtaboeuf, France.
†hsp60, heat shock protein 60; gyrB, DNA gyrase subunit B.

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