Volume 16, Number 12—December 2010
Dispatch
Leishmania tropica Infection in Golden Jackals and Red Foxes, Israel
Dalit Talmi-Frank, Noa Kedem-Vaanunu, Roni King, Gila Kahila Bar-Gal, Nir Edery, Charles L. Jaffe, and Gad Baneth
Table
Sequence similarity obtained for Leishmania spp. ITS1-positive tissue samples from jackals and foxes, Israel*
| Subject no. | Animal species | Tissue | HRM results | ITS1 sequence length (% similarity†) | GenBank accession nos. |
|
|---|---|---|---|---|---|---|
| Comparison isolate | Identified isolate | |||||
| 918 | Jackal | Right ear, left ear | L. tropica | 236–239 bp (98) | FJ948456 | GU591390 |
| 922 | Jackal | Right ear, left ear | L. tropica | 235–239 bp (98) | FJ948456 | GU591391 |
| 1067 | Jackal | Right ear, spleen | L. tropica | 233–237 bp (98) | FJ948456 | GU591392 |
| 1086 | Jackal | Right ear, spleen | L. tropica | 234–238 bp (98) | FJ948456 | GU591393 |
| 1380 | Jackal | Spleen | L. tropica | 235–239 bp (98) | FJ948456 | GU591394 |
| 115 | Jackal | Blood | L. infantum | 221–222 bp (99) | GU045592 | GU591395 |
| 1084 | Fox | Right ear, snout, spleen | L. tropica | 234–239 bp (98) | FJ948456 | GU591396 |
| 579916 | Fox | Left ear | L. tropica | 235–239 bp (98) | FJ9484556 | GU591397 |
*ITS1, internal transcribed spacer 1; HRM, high-resolution melt analysis.
†Similarity to comparison isolate by BLAST (www.ncbi.nlm.nih.gov/BLAST).
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